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Full Description
This volume serves as an indispensable guide for researchers, students, and professionals who wish to explore the intricate world of pollen biology and its modern methodologies. Chapters detail collecting, counting, storing pollen, artificial intelligence, pollen classification, flow cytometric analysis, pollen characteristics, multispectral imaging, pollen tube growth in stiff media and measuring self-incompatibility in plants, providing solutions and methodologies for these obstacles. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and cutting-edge, Pollen and Pollen Tube Analysis: Methods and Protocols represents a collaborative effort to advance the understanding of pollen biology using modern techniques and applications. This volume will serve as a valuable resource for those entering the fascinating field of pollen research.
Contents
Assessment of pollen viability and germination through histochemical, in vivo and in vitro methods.- Evaluating pollen viability and tube growth in rice (Oryza sativa).- Analysing pollen with multispectral imaging flow cytometry (MIFC).- Pollen and pollen tube sampling and observation by scanning electron microscopy and transmission electron microscopy.- Visualizing pollen and pollen tube development using fluorescence microscopy.- Automated measurements of fluorescence in spatially defined regions of pollen tubes.- Quantitative profiling of metabolites, sugars, and lipids in pollen tubes by mass spectrometry.- Analyzing pollen by Infrared and Raman spectroscopy.- Extracting and quantifying specific pollen proteins by Western blot.- Detecting ROS production by pollen grains and tubes.- Quantitative phenotyping of ion fluxes in tip-growing cells under varying growth regimes: a data analysis protocol.- Quantitatively analyzing the structural organization at the tip of growing tobacco pollen tubes.- Implementing in vitro and in vivo approaches to analyze lipid-protein interactions in growing tobacco pollen tubes.- Evaluation of pectin and arabinogalactan protein distribution in olive pollen tube cell walls using immunofluorescent labelling.- Evaluating self-incompatibility in citrus by histological and molecular assays.- Comparing in vivo and in vitro pollen tube attraction assays for Arabidopsis and maize.- Employing biolistic transformation to investigate subcellular protein localization and interactions in growing tobacco pollen tubes.- Isolation of proteins and extracellular vesicles secreted by pollen of Actinidia chinensis L. during in vitro germination.- Preparing a pollen reference collection and standardised protocols for aerobiological and melissopalynological pollen slides.- Palynology: extracting pollen from mosses and sediments.
