- ホーム
- > 洋書
- > 英文書
- > Gardening & Plants
Full Description
This volume aims to provide a comprehensive collection of modern experimental techniques, protocols, and analytical tools for studying plant antiviral resistance. Chapters detail viral infection, plant immunity, advanced methodologies for high-throughput screening, omics-based profiling, and cutting-edge proximity tagging technology applications. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and authoritative, Plant Antiviral Resistance: Methods and Protocols is a valuable resource for researchers and scientists who want to learn more about this important and developing field.
Contents
Development of Tobamovirus-Resistant Tomato Plants by CRISPR-Cas9-Mediated Knockout of Susceptibility Genes.- Silencing host factors with hairpin RNA for virus resistance.- Heritable Tissue-Culture-Free Gene Editing in Nicotiana benthamiana Using a Meristem-Invading Virus Vector.- Pepper veinal mottle virus (PVMV)-based vector for expressing pathogen-derived resistance in pepper.- Construction of Attenuated Viruses via Changing the UpA Dinucleotide Frequency.- Mild strain cross-protection in passion fruit.- A TurboID-based protocol for efficient interacting proteins identification using proximity tagging technology.- Protocol for Exogenous Jasmonate Treatment and Quantitative Assessment of Plant Virus Infection.- Exogenous melatonin application via foliar spray and root irrigation for plant virus control.- Phosphatidic acid application to enhance plant virus resistance.- Virus elimination by thermotherapy combined with meristem culture.- Combining chemical treatment with shoot tip culture for plant virus eradication in fruit trees.- Design Artificial Resistance Using Plant Elicitor Peptides.- Virus-induced NLR-silencing library-based screening to identify antiviral NLR genes.- VIGS-based screening for NLR genes regulating viral effector-induced hypersensitive response.- High-Throughput Screening of Antiviral Agents Using the Fluorescence-Based Local Lesion Inspection Assay (FLLIA).- Enhancing Plant Antiviral Resistance with Microbial Immunomodulators: A Half-Leaf Spot Screening Method.- Screening and identification of antiviral chemical components from medical plants.- Development of a reverse genetics system for rice tiller inhibition virus.- Methods for Autophagy Detection by Fluorescence Microscopy.
