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Full Description
This volume presents methods on cereal breeding, double haploid(DH) production, and protocols for generating DH lines for breeding programs. Chapters guide readers through challenges in cereal breeding, plastid function, gene regulation, chloroplast development, Imperata cylindrica, hybridization of bread wheat with barley, and methodologies to examine changes in cellular organization and for localizing key molecular determinants through immunofluorescent assays within the context of ME. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and thorough, Doubled Haploid Methods in Cereals aims to present up-to-date, reproducible approaches to inspire and empower researchers and breeders to develop, refine, and implement DH systems across diverse cereal species.
Contents
Current Challenges in Cereal Breeding.- Molecular Insights into Microspore Embryogenesis: Transcriptomic Dynamics in Barley and Wheat.- Albinism in Cereal Androgenesis - Shedding Light on Molecular Mechanisms and Possible Solutions.- Production of Wheat Doubled Haploids Through Intergeneric Hybridization.- Wheat Doubled Haploid Production Through Maize Crossing.- Bread Wheat (Triticum aestivum L.) Doubled Haploid Production by Interspecific Crosses with Imperata cylindrica.- Hexaploid Wheat x Barley (Triticum aestivum x Hordeum vulgare) Intergeneric Hybrids: Production and Analysis.- Bread Wheat Anther Culture.- Bread Wheat Microspore Culture.- Durum Wheat Anther Culture: A Complete Protocol.- Barley Doubled Haploid Plant Production by Anther Culture.- Monitoring Barley Microspore Embryogenesis: In Vitro Culture and Immunolocalization Analyses.- Production of Homozygous Maize Lines using Maternal Haploid Inducer lines.- In Vitro Anther Culture of Japonica and Indica Rice (Oryza sativa L.).- Indica Rice Anther and Shed Microspore Culture.- Isolated Microspore Cultures of Triticale (xTriticosecale Wittm).- Efficient Protocol for Doubled Haploid Production in Rye (Secale cereale L.).- Targeted Gene Editing in Wheat during Haploid Production via Wide Hybridization with Transgenic Maize Expressing Cas9 and Guide RNA.- In Vivo Rice Haploid-induction System by an Egg Cell-specific Peptidase Knockout.
