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Full Description
This detailed collection covers methods recently developed for experiments on organisms from each of the major euglenozoan groups, including diplonemids, euglenids, and kinetoplastids. Volume 2 presents three sections exploring techniques in molecular biology, cell biology, as well as biochemistry and physiology. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.
Authoritative and practical, Euglenozoa: Methods and Protocols, Volume 2 serves as an ideal guide for researchers working with these fascinating organisms and investigating the aspects of their extreme biology.
Chapters 21 and 22 are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
Contents
Genetic Manipulation of Paradiplonema papillatum.- Protocol for Efficient Ribodepletion of Euglena gracilis RNA.- Engineering Wax Ester Composition in Euglena gracilis Using Genome Editing.- Multi-Approach Workflows for Chromatin and Histone Isolation from Trypanosoma cruzi: From Basic Protein Extraction to Mass Spectrometry-Compatible Protocols.- Precision Without Selection: A Marker-Free CRISPR/Cas9-Based Protocol for Multiplexed Genome Editing in Trypanosomatids.- Application of Double-Inducible Expression System in Bloodstream-Form Trypanosoma brucei.- Tetracycline-Inducible Functional Complementation Analysis in Trypanosoma Parasites.- Immunoprecipitation of Protein Complexes from Trypanosoma brucei.- Standardized Recombinase Polymerase Amplification (RPA) Assay for Detecting Active Trypanosoma brucei evansi Infections.- High Throughput Cell Cycle and Morphological Analysis of Leishmania mexicana and Other Kinetoplastids.- Protein Localization with Streptavidin-Imaging: Boost Your Signal, Reduce Noise, Overcome Antibody Accessibility Problems, and Time-Travel to the Past.- A Practical Guide to Ultrastructural Expansion Microscopy (U-ExM) in Trypanosoma brucei.- Expansion Microscopy in Euglenozoa.- Colab-PEX19BS: An Automated Google Colab Notebook-Based Tool for Detecting PEX19 Binding Sites.- Biochemistry of Diplonemids.- Euglena gracilis Subcellular Fractionation.- Isolation of Mitochondria and Chloroplasts from Euglena gracilis Z.- Imaging Ca2+ Signaling in Trypanosoma cruzi with Genetically-Encoded Ca2+ Indicators.- Enzyme Assays for Phosphoinositide Kinases and Phosphatases and Inhibitor Screens.- Non-Invasive, Fluorescence-Based Detection of Perturbations in the Thiol-Redox Homeostasis of Bloodstream Trypanosoma brucei brucei.- Assessment of Mitochondrial Membrane Potential in Intact and Detergent-Permeabilized Trypanosoma brucei Insect and Bloodstream Forms.- Camelid Single-Domain Antibodies as Tools for Novel Target Identification in Kinetoplastid Research.- Method for Purification and Electrophysiological Recordings of Ion Channels from Trypanosomatids.- Functional Studies of Kinetoplastid Transporters.
