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Full Description
This third edition expands upon the chapters of the previous editions, providing new and updated methods. Chapters explore protein-lipid interactions, membranes, membrane protein structure, how membrane proteins affect lipids and how they are in turn affected by the lipid bilayer, and lipid properties. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and authoritative, Lipid-Protein Interactions: Methods and Protocols, Third Edition is an essential resource for all researchers who are interested in obtaining up-to-date and comprehensive information about membrane structure and function.
Contents
Characterization of membrane binding and protein-lipid interactions at atomic level with accelerated HMMM model.- Quartz Crystal Microbalances as Tools for Probing Protein-Membrane Interactions.- Surface Plasmon Resonance for Measuring Interactions of Proteins with Lipids and Lipid Membranes.- Isothermal Titration Calorimetry for Investigating Thermodynamics of Protein-Lipid Interactions.- Differential Scanning Calorimetry of Protein-Lipid Interactions.- Purification of Bacterial Lipopolysaccharide, and how to study its interactions with proteins.- Analysis of the Kinetics of Folding and Insertion of β-Barrel Membrane Proteins by Gel-Electrophoresis.- Imaging Lipid-Related Macromolecular Structures Using Optimized Negative Staining Transmission Electron Microscopy.- Probing the Annular Lipid belt of Membrane Proteins Using Native Mass Spectrometry.- Protein Microarrays and Liposome: A Method for Studying Lipid-Protein Interactions.- Complex Structural Examination of Protein-Lipid Interactions with Neutron Scattering Techniques.- Circular-Dichroism and Synchrotron-Radiation Circular-Dichroism Spectroscopy as Tools to Monitor Protein Structure in a Lipid Environment.- Protein-Membrane Interactions Studied by Transmission and Total Internal Reflection FTIR Spectroscopy.- Deep UV Resonance Raman Spectroscopy: Applications to Membrane Proteins and Peptides.- Analyzing Transmembrane Protein and Hydrophobic Helix Topography by Dual Fluorescence Quenching.- Förster Resonance Energy Transfer as a Tool for Quantification of Protein-Lipid Selectivity.- A Guide to Tracking Single Membrane Proteins and Their Interactions in Supported Lipid Bilayers.- Fluorescence Correlation Spectroscopy to Examine Protein-Lipid Interactions in Membranes.- Membrane Pore Formation by Peptides Studied by Fluorescence Techniques.- Sited-directed fluorescence spectroscopy for studies on the folding and insertion of proteins in lipid bilayers and model membranes.- EPR Techniques to Probe Insertion and Conformation of Spin-Labeled Proteins in Lipid Bilayers.- Electron paramagnetic resonance spectroscopy of spin-labeled lipids for studying lipid-protein interaction in model- and biomembranes.- Non-linear continuous wave electron paramagnetic resonance spectroscopy for investigation of lipid-protein interactions in membranes.
