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Full Description
This fully updated edition examines recent advances in algal biotechnology and incorporates both foundational and newly developed protocols for working with algal cultivation systems and biomass. Guiding the reader from initial cultivation to advanced biochemical analysis, the book covers the rationale for using algae in biofuel production and considerations for selecting appropriate strains and cultivation systems, protocols for quantifying key biochemical components of algal biomass, including carbohydrates, lipids, and proteins, as well as what to consider when developing an algal industry such as its environmental impact and what tools and methods can be used in the continued development of this field. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.
Authoritative and up-to-date, Biofuels from Algae: Methods and Protocols, Second Edition serves as an ideal guide for making algal biofuel research more accessible.
Contents
Basic Methods for Isolating and Culturing Microalgae.- Measurement of Fluorescence for Monitoring Algal Growth and Health.- Large Scale Cultivation of Microalgae in Open, Closed, and Hybrid Systems.- Growing Algae in Biofilms: Principles and Emerging Opportunities.- Combining Aquaponics and Applied Phycology: From Bioremediation of Wastewater to Algal Products.- Monitoring Microalgal Neutral Lipid Accumulation with Nile Red.- Liquid Chromatography-Mass Spectrometry (LC-MS)-Based Analysis of Molecular Lipids in Algae Samples Using Data Dependent Acquisition (DDA).- UPLC-ELSD Analysis of Algal Lipid Classes and Derivatization of Bound and Free Fatty Acids and Sterols for GC-MS Methods.- Total Fatty Acid Content Determination of Whole Microalgal Biomass Using In Situ Transesterification.- Total Carbohydrate Content Determination of Microalgal Biomass by Acid Hydrolysis Followed by Spectrophotometry or Liquid Chromatography.- Total Protein Content Determination of Microalgal Biomass by Elemental Nitrogen Analysis and a Dedicated Nitrogen-to-Protein Conversion Factor.- Complete Acid-Based Hydrolysis Assay for Carbohydrate Quantification in Seaweed: A Species-Specific Optimized Approach.- Analysis of Brown Algal Sugars Using Ion-Exchange High-Performance Liquid Chromatography.- Chromatographic Purification of Fucoxanthin from Diatom Biomass.- Analytical Grade Purification of Phycocyanin from Cyanobacteria.- Phytoplankton Pigment Analysis by High Performance Liquid Chromatography.- Extracellular Enzyme Activity Measurements Using Fluorescent Substrate Derivatives.- Determining Inorganic and Organic Carbon.- Determining Inorganic and Organic Nitrogen.- Total Nitrogen Determination by a Spectrophotometric Method.- Determining Inorganic and Organic Phosphorus.- Determining Dissolved and Biogenic Silica.- Microalgae Harvesting Using Fungal Mycelia in Suspension.- RNA Extraction Strategy for Algae Rich in Polysaccharides and Polyphenols.- A Simple Staining Method to Study Cell Structures of Microalgae.- Life Cycle Assessment of Micro and Macro Algae Products.- Life Cycle Assessment (LCA) of Algal Fuels.- Design and Analysis of Macroalgae-Based Biorefineries.- Metabolic Engineering of Microalgae for Biofuel Production.
