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Full Description
This groundbreaking volume dives into the devastating impact of infections on the Central Nervous System (CNS), a major culprit behind neurological disorders in both children and adults. From bacteria and viruses to fungi and parasites, a range of pathogens can breach the body's defenses and wreak havoc on the brain. Packed with cutting-edge research, this book unravels the methods and tools used to study how these microbial invaders penetrate human cell barriers, interact with CNS cell types—especially neurons and microglia—and contribute to lasting brain damage. As part of the acclaimed Methods in Molecular Biology series, each chapter provides a clear introduction, essential materials and reagents, step-by-step lab protocols, and expert troubleshooting tips to ensure reproducible results.
Whether you're a seasoned researcher or just stepping into the field, In Vitro and In Vivo Models to Study Infections of the Central Nervous System is your go-to guide for exploring this critical and rapidly evolving area of study.
Contents
Studying Group B Streptococcus-Blood-Brain Barrier Interaction In Vitro using an Induced Pluripotent Stem Cell Derived Brain-Like Endothelial Cell Model.- E Coli. Meningitis.- Neuro-Immune Consequences of L. Monocytogenes Infection.- In Vitro and In Vivo Models Studying Haemophilus Influenzae.- A 3D In Vitro Blood-Brain Barrier Model to Study Pathogen and Drug Effects on the BBB.- In Vitro Culture of Primary Mouse Neurons to Study Neuronal Infection.- Differentiation of Human Neurons and Neuron-Like Cells to Study VZV Neuronal Infection.- Enterovirus Meningitis.- Lab Protocols for Coxsackievirus B3 Infection Using Brain-Like Endothelial Cells Derived from Induced-Pluripotent Human Stem Cells.- Infection of Macrophages with Cryptococcus neoformans to Evaluate Dissemination to the Central Nervous System.- Infection of Mice with Cryptococcus neoformans to Evaluate Dissemination to the Central Nervous System.- In Vitro and In Vivo Models of Neuroschistosomiasis.