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Full Description
This updated collection features protocols that reflect the continued expansion of stem cell niche research. These specialized microenvironments that regulate stem cell function have continued to inspire tremendous interest as a subject of study in the years since the publication of the second edition. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Stem Cell Niche: Methods and Protocols, Third Edition serves as an ideal guide for both experts and novices in the stem cell field.
Contents
Human Skeletal Muscle Niche Formation and Analysis with Spatial RNA Sequencing.- Three-Dimensional Analysis of Skeletal Muscle Stem Cell Niche Following Tissue Clearing.- Efficient Isolation and Ex Vivo Differentiation of Murine Satellite Cells from Healthy and Dystrophic Muscle.- Isolation of Mouse Bone Marrow Niche Cells for Single Cell Sequencing.- Microwells as Minimalistic Niches to Study Heterotypic Interactions of Stromal and Hematopoietic Stem Cells.- Detection of Cellular Senescence on Murine Muscle Tissue Sections by Senescence-Associated β-Galactosidase Staining.- Light-Sheet Microscopy Enables Three-Dimensional Fluorescence Imaging and Live Imaging of Satellite Cells on Skeletal Muscle Fibers.- Stem Cell Niche: iPSC-Based Assembloids for Modeling Human Hematopoiesis.- Oligopaint FISH in Drosophila Testes.- Evaluation of Adult Mouse Brain Neurogenic Niche Behavior by Brain Slices In Vitro Culture Method.- Isolation of Murine Neonatal and Adult Osteomacs to Examine Their Role in the Hematopoietic Niche.- Telocytes: Detection, Visualization, Tissue Dissociation, and Tamoxifen-Induction of Transgenic Mice.- Co-Colonization and Co-Culture of Lung Alveoli Epithelial Stem Cells and Their Endothelial Niche Cells.- Simplified Quantification of Progenitor Zone Size, an Indicator of Germ Stem Cell Niche Activity, in the Nematode Caenorhabditis elegans.- Measurement of Myonuclear Accretion In Vitro and In Vivo.- A Simple Method to Dissect, Orientate, and Visualize the Murine Limbal Stem Cell Niche with Cornea and Conjunctiva Attached.- Regeneration of Volumetric Muscle Loss Using MSCs Encapsulated in PRP-Derived Fibrin Microbeads.- Standardizing Chondrocyte Isolation and Articular Cartilage Decellularization: A Versatile Bioink for Tissue Engineering Applications.- Electrospinning Approach to Preparing Nanostructured Scaffolds for Stem Cell Seeding and/or Implantation in Neurotrauma.- Development of a Three-Dimensional Multicellular Model of Human Neuroblastoma Using Matrigel as an Extracellular Matrix Analogue.- Principles of Indirect Co-Culture Method Using Transwell.- Upstream Process Protocol for MSCs Isolated from Different Human-Based Tissue Origins.- Isolation and Culturing of Newborn Rat Nasal Chondrocytes.
