Full Description
B-lymphocytes produce antibodies, mediate humoral responses, and act as a key to the adaptive immune system of our body. The development and function of B cells are largely dependent on B cell antigen receptors (BCRs), which transmit a multitude of signals upon binding to the cognate antigen and activate the survival, proliferation, and differentiation of B cells thereof. Thus, studying the BCR signaling remains fascinating for both basic and translational research. This volume provides a collection of protocols ranging from classical cell and molecular biology applications to state-of-the-art omics techniques for studying BCR signaling. Written in the highly successful Methods in Molecular Biology series format, chapters detail the strengths, weaknesses, and troubleshooting techniques that we hope will be helpful to researchers.
Cutting-edge and thorough, B-Cell Receptor Signaling: Methods and Protocols will inspire immunologists in designing new experiments to explore the classics from a different perspective, as well as ask some stunning biological questions of our time.
Contents
Adoptive Transfer of B Cells In Vivo for Assessment of Their Immune Function.- Analysis of Germinal Center Reaction in Competitive Bone Marrow Chimeric Rag2-/-γc-/- Mice.- Immunophenotyping of B Cell Subpopulations by Flow Cytometry.- Evaluation of Constitutive or Induced Activation State of the VLA-4 Integrin in Human and Murine Samples.- Traction Force Microscopy for Studying B Lymphocyte Mechanosensing.- High-Resolution Imaging of Intracellular Trafficking of B Cell Receptor Using Specific Hybridization Internalization Probe (SHIP).- In-Depth, High-Throughput Analysis of BcR IG Clonality and Diversity Levels in Immune Repertoires.- Recombinant Production of B-Cell Receptor Fab Fragments from Chronic Lymphocytic Leukaemia in Mammalian Cells for Structural and Biochemical Studies.- Design, Production, and Optimization of Antigen-Specific Recombinant Antitumor Dimeric IgA Antibody.- Facilitating Gene Editing in Human Lymphoma Cells Using Murine Ecotropic γ-Retroviruses.- CRISPR/Cas9 Methods for Identification and Validation of Genes Regulating BCR-Mediated Antigen Uptake.- A Systems Biology Approach of Quantifying Signal Transduction to B Cell Proliferation and Differentiation.- Comparative Profiling of Regulatory Modules as a Tool for Identifying the Transcription Factor Network Linked to Leukemogenesis.- Studying Mitogen-Independent Proliferation in Murine B Cells.- B Cell Co-Culture in the Tumor Microenvironment of Solid Tumors Using Pancreatic Cancer as a Tumor Model.- A Comprehensive Methodology for Immortalizing Tumor-Infiltrating B Lymphocytes from Epithelial Cancers.- Quantification of Telomere Length in Peripheral Blood Mononuclear Cells Using Quantitative Polymerase Chain Reaction.- Generation and Reactivity Profiling of Functional Human Recombinant Monoclonal Antibodies.
