Full Description
This detailed volume collects diverse experimental approaches essential for researchers exploring the intricacies of cardiomyocyte biology and pathology. The book features basic isolation methods, techniques for studying specific cellular processes and responses, protocols that bridge in vitro studies with in vivo models, all of which are vital for delving into the complexities of cardiomyocyte biology and its implications for cardiovascular health and disease. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Cardiomyocytes: Methods and Protocols serves as a valuable resource for both novice and seasoned researchers in the field of cardiomyocyte research.
Contents
Isolating and Culturing Neonatal Cardiomyocytes.- Simple Method to Isolate Individual Mouse Cardiomyocytes Using Enzymatic Digestion via Retrograde Perfusion.- Isolation of Endothelial Cells from Human Internal Mammary Artery.- Mitochondrial Membrane Potential and Lactate Dehydrogenase Activity in a Model of Acute In Vitro Ischemia-Reperfusion Injury.- Induction on Insulin Resistance In Vitro.- Preparation of Human Atrial Trabeculae for Ex Vivo Analysis of Human Myocardial Function.- Measurement of Intracellular Ca2+ in Muscle Isolated from Rat and Human Hearts.- Pressure Myography.- Langendorff Isolated Heart Perfusion Model for Functional Studies in Mice.- Exosome Isolation from Pericardial Fluids.- Quantitative Real-Time PCR for Circular RNA Detection and Analysis.- Identification and Analysis of Small Nucleolar RNAs by Real Time Quantitative PCR.- Extraction of Total Protein from Cardiomyocytes and Western Blotting Analysis.- Cationic Liposome Formulation by Microfluidics for miRNA Delivery.- Quantification of Sympathetic Nerve Traffic to the Heart: Electrophysiological Recording of Cardiac Sympathetic Nerve Activity in a Rodent Model.- An Ovine Model of Chronic Heart Failure Induced by Repeated Coronary Microembolizations.
