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Full Description
This volume explores the latest advancements in the study of the Marburg virus. The first few chapters in this book review the challenges associated with developing effective medical countermeasures against this virus, while the rest of the chapters provide protocols on ways to achieve these goals. Some of the topics covered in this book include virus isolation and propagation; single-cell transcriptional profiling; reverse genetics systems; proteomics analyses; next-generation sequencing techniques; protocols detailing the use of various animal models including bats and nonhuman primates; and methods for assessing virus stability or validating virus inactivation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and thorough, Marburg Virus: Methods and Protocols is a valuable resource for all researchers interested in advancing their understanding of this virus.
Contents
Mismatch of Supply and Demand: Marburg Virus Disease Outbreaks Need Countermeasures but Will Not Provide Opportunity for Clinical Trials.- Marburg Virus Medical Countermeasures.- Isolation and Propagation of Marburg viruses.- Validation of Marburg Virus Inactivation by AVL Buffer.- Generation, Recovery, and Propagation of a Recombinant Vesicular Stomatitis Virus Expressing the Marburg Virus Glycoprotein.- Visualizing the Internalization of Marburg Virus-Like Particles into Living Cells.- Proximity Proteomics to Profile Ebola Virus Protein Interactome in its Functional Context.- Monoclonal Antibody Production Against Filoviruses from Immunized Mice.- Marburg Virus Minigenome Assays.- Transcription- and Replication-Competent Virus-Like Particle Systems for Marburg Virus.- Generation of Biologically Contained Marburg Virus.- Recovery of Recombinant Marburg Virus by Reverse Genetics.- In Vivo Investigation of Filovirus Glycoprotein-Mediated Infection in a BSL2 Setting.- Evaluating Countermeasures against Marburg Virus Using a Mouse Model.- Filovirus Infection in Humanized Mouse Models.- A Hamster Model for the Evaluation of Marburg Virus Countermeasures.- Evaluation of Marburg Virus Medical Countermeasures in Guinea Pigs.- Experimental Infection of Egyptian Rousette Bats (Rousettus aegyptiacus) with Marburg Virus.- Evaluation of Vaccines and Therapeutics Against Ebolaviruses in the Domestic Ferret.- Evaluation of Vaccines and Therapeutics against Marburg Virus in Nonhuman Primate Models.- Marburg Virus Aerosol Infection in Animal Models.- Gross and Histopathologic Evaluation of Tissues from Marburg Virus Infected Nonhuman Primates.- Detection of Serum Antibodies Targeting the Marburg Virus Glycoprotein using a Multiplex Immunoassay Platform.- Quantification of Neutralizing Antibodies in Serum using VSV-MARV-GFP.- Cellular Phenotyping of Peripheral Blood Mononuclear Cells from Marburg Virus-Infected Animals.- Transcriptional Profiling of Filovirus-Infected Nonhuman Primate Samples.- Droplet-Based Single Cell 3' mRNA Sequencing of Marburg Virus Infected Samples.- Quantification of Filovirus Soluble Glycoprotein using a Sandwich ELISA.- Immunochromatographic Lateral Flow Assays to Detect Filovirus Nucleoproteins.- Amplification of Filovirus Genomes from Clinical Samples for Next Generation Sequencing.- Virus Stability on Surfaces, in Bodily Fluids, in Wastewater, and Different Environmental Conditions.
