Full Description
This detailed volume presents state-of-the-art techniques in ocular regeneration research, pulling together recent animal model systems identifying key genes and regulatory networks that contribute to the regenerative response as well as advances in induced pluripotent stem cell (iPSC) biology. The book opens with a section on the anterior segment and key models and techniques utilized to study the cornea, lens, and trabecular meshwork, and continues with chapters on the posterior segment, and largely the retina and retinal pigment epithelium (RPE), with techniques developed in a variety of model systems to study regeneration of these tissues. The concluding part examines novel technologies utilized in ocular regeneration research. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Ocular Regeneration: Methods and Protocols serves as an ideal guide for researchers aiming to expand the boundaries of what we know about this vital area of physiology.
Contents
Isolation, Culture, and Quality Assessment of Clinical-Grade Corneal Stromal Stem Cells.- Generation of Functional Lentoid Bodies from Human Induced Pluripotent Stem Cells.- Lens Regeneration: The Application of iSyTE and In Silico Approaches to Evaluate Gene Expression in Lens Organoids.- Trabecular Meshwork Regeneration for Glaucoma Treatment Using Stem Cell-Derived Trophic Factors.- Models of Photoreceptor Degeneration in Adult Zebrafish.- Isolation and Preparation of Embryonic Zebrafish Retinal Cells for Single-Cell RNA Sequencing.- Nuclei Isolation from Ocular Tissues of the Embryonic Chicken for Single-Nucleus Profiling.- Experimental Framework for Assessing Mouse Retinal Regeneration through Single Cell RNA-Sequencing.- Isolation and Characterization of Extracellular Vesicles to Activate Retina Regeneration.- In Vivo Imaging of Rodent Retina in Retinal Disease.- Retinal Explant Culture from Mouse, Human, and Non-Human Primates and Its Applications in Vision Research.- Generating ESC-Derived RGCs for Cell Replacement Therapy.- Generation of Induced-Primary Retinal Pigment Epithelium from Human Retinal Organoids.- Automated Phenotypic Screening Platform for Identifying Factors that Affect Cell Regeneration Kinetics In Vivo.- Chromatographic Purification and Polishing of AAV Particles.- Controlled Release of Molecules to Enhance Cell Survival and Regeneration.- Development and Validation of a Proximity Labeling Fusion Protein Construct to Identify the Protein-Protein Interactions of Transcription Factors.
