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Full Description
This volume explores the latest experimental and computer-based methods used to study the field of motility research. The chapters in this book cover topics such as methods to investigate motility and chemotaxis in a range of systems including macrophages and Dictyostelium discoideum; ways in which electric fields impact motility and taxis; recently developed computer-based methods for studying pseudopod-driven locomotion of amoeboid cells; and software tools for kymograph-based analysis of cell contour dynamics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and practical, Cell Motility and Chemotaxis: Methods and Protocols is a valuable resource for researchers looking to expand their knowledge of motility-related research across different disciplines.
Contents
Chemotaxis Assay of Bone Marrow-Derived Macrophages.- Methods and Protocol for Single Cell Motility Assays under Topological Constraints.- Imaging-Based Analysis of Cell-Cell Contact Dependent Migration in Dictyostelium.- A Mutant of Dictyostelium discoideum, KI-Cell, As a Model of Collective Cell Migration Independent of Chemotaxis.- Using Multiphoton Intra-Vital Microscopy to Study Neutrophil Transmigration and Blood-Brain Barrier Permeability in a Mouse Model of Herpes Simplex Virus Encephalitis.- An In Vivo Metastasis Model using Genotype-Defined Tumor Organoids.- Different Morphotypes of Physarum polycephalum as Models for Chemotaxis and Locomotion.- Flagella and Cell Body Staining of Bacteria with Fluorescent Dyes.- Re-Purposing Proximity-Dependent Protein Labelling (BioID2) for Protein Interaction Mapping in E. coli.- Electrotaxis of Dictyostelium discoideum, Migration in an Electric Field.- Electric Cell-substrate Impedance Sensing as a Tool to Characterize Wound Healing Dynamics.- Chemotaxis of Large Multinucleate Cells of Dictyostelium Produced by Electric-Pulse Induced Fusion.- A Hands-On Guide to AmoePy: A Python-Based Software Package to Analyze Cell Migration Data.- Pseudopod Tracking and Statistics during Cell Movement in Buffer and Chemotaxis.- Generation of a Virtual Cell using a Phase Field Approach to Model Amoeboid Crawling.
