Full Description
This volume covers the classic and new techniques used to study the zoonotic pathogen Streptococcus suis. The chapters in this book cover are organized in four sections and cover topics such as techniques for cultivation, isolation, typing of S. suis biofilm formation and genetic manipulation; analysis of antibiotic resistance and transference; detection in pigs and infection models; and evaluation of economic impact and prevention. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and thorough, Streptococcus suis: Methods and Protocols is a valuable resource for both novel and expert researchers who want to expand their knowledge of this important field.
Contents
Laboratory Methods for Culture and Identification.- In Silico Typing and Identification Confirmation of Streptococcus suis Isolates.- Formation and Analysis of Streptococcus suis Biofilm In Vivo.- Techniques for Genetic Manipulation of Streptococcus suis.- Analysis of Susceptibility or Resistance to Antimicrobial Agents.- Detection of Macrolide and/or Tetracycline Genetic Resistance Determinants by PCR.- Detection and Quantification of Conjugative Transfer of Mobile Genetic Elements Carrying Antibiotic Resistance Genes in Streptococcus suis.- Analysis of the Nasal Microbiota in Health and Disease.- Experimental Intraperitoneal Infection of Piglets with Streptococcus suis.- Evaluation of the Economic Impact of Streptococcus suis-Associated Disease.- Preparation and Study of Bacterins against Streptococcus suis.
