Full Description
This volume discusses the latest approaches used to investigate molecular biology and physiology in the peripheral and central olfactory system. Understanding both normal function and pathobiology provides readers with insight into understanding human olfactory disorders. The chapters in this book cover topics such as techniques for manipulating and measuring olfactory cilia function; approaches for investigating spatial RNA expression in olfactory mucosa; the use of chromatin immunoprecipitation with olfactory neurons; in vivo methods to label and identify activated olfactory neurons; defining organization in the olfactory bulbs and cortex; and the use of optical recording in the mouse olfactory bulb for acute or chronic experiments. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Thorough and cutting-edge, The Olfactory System: Methods and Protocols is a valuable resource for all researchers who are interested in learning more about this important and developing field.
Contents
Visualizing and Manipulating Olfactory Cilia through Viral Delivery Coupled with En Face Imaging of Intact OE.- Genome-Wide RNA Tomography in the Mouse Whole Olfactory Mucosa.- Optical Activation of Photoswitchable TRPC Ligands in the Mammalian Olfactory System Using Laser Scanning Confocal Microscopy.- Intranasal Pressure Recording for Monitoring Mouse Respiration.- Identification of Immune Cells in Murine Olfactory Mucosa.- Chromatin Immunoprecipitation from Formaldehyde Cross-Linked Olfactory Sensory Neurons.- Activity-Dependent Labeling of Olfactory Sensory Neurons using RNA Fluorescence In Situ Hybridization followed by Phospho-S6 Immunofluorescence.- Measuring Cell Surface Expression of Odorant Receptors Via Flow Cytometry.- Dissociation of Mouse Olfactory Mucosae for Fluorescence-Activated Cell Sorting of Olfactory Sensory Neurons.- Preparation of Human Olfactory Epithelial Biopsies for Downstream Analysis.- Cranial Window for Acute and Chronic Optical Access to Record Neuronal Network Dynamics in the Olfactory Bulb.- Target-Captured mRNA from Murine Olfactory Bulb.- Identification and Localization of Cell Types in the Mouse Olfactory Bulb using Slide-SeqV2.- Dense and Sparse Labelling of Mitral Cells by Oral and Intraperitoneal Routes of Tamoxifen Administration.- Techniques in Staining of Rodent and Human Olfactory Tissue.- Electrophysiological Recordings from Identified Cell-Types in the Olfactory Cortex of Awake Mice.
