Full Description
This detailed volume provides various cellular and experimental animal models and research methodologies to understand the complex interrelated pathophysiological mechanisms triggered by hyperglycemia that underlie the development of diabetic retinopathy. The book covers methods for understanding the pathogenesis of diabetic retinopathy, detection and screening of biomarkers in diabetic retinopathy, as well as novel technologies for diabetic retinopathy management. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Diabetic Retinopathy: Methods and Protocols serves as an invaluable resource for scientists and clinicians who are interested in the area of ophthalmology research.
Contents
Induction of Type 2 Diabetes in Mice to Understand Vascular Changes that Drive Diabetic Retinopathy.- Akimba Proliferative Diabetic Retinopathy Model: Understanding Molecular Mechanism and Drug Screening for the Progression of Diabetic Retinopathy.- Oxygen-Induced Retinopathy in the Rat: An Animal Model to Study the Proliferative Retinal Vascular Pathology.- Retinal Assessment Using In Vivo Electroretinography and Optical Coherence Tomography in Rodent Models of Diabetes.- A Guide to Genome Wide Association Study Design for Diabetic Retinopathy.- Single Cell RNA Sequencing (scRNA-Seq) Data Analysis of Retinal Homeostasis and Degeneration of Microglia.- Bioinformatics Tools for Bulk Gene Expression Deconvolution in Diabetic Retinopathy.- MicroRNA Profiling from Tears as a Potential Non-Invasive Method for Early Detection of Diabetes Retinopathy.- Proximity Extension Assay (PEA) Platform to Detect Vitreous Biomarkers of Diabetic Retinopathy.- Methods for Analyzing the Gut Microbiota in Diabetic Retinopathy.- Mass Spectrometry-Based Vitreous Proteomics: Validated Methods and Analysis Pipeline.- Methods for Ex Vivo AAV Transduction in Rodent and Human Retinal Explants.- Methods for RPE Sheet Construction Using iPS-CM in Conjunction with Natural Scaffold of Corneal Lenticule Derived from SMILE.- Chromatin Immunoprecipitation Assay in Mouse Retinal Tissue.- Frozen Block Tissue Staining for Eye Structure of Zebrafish Embryo.- Investigating the Function of MicroRNAs in Human Retinal Microvascular Endothelial Cells of Diabetic Retinopathy.- Genome Editing Inhibits Retinal Angiogenesis in a Mouse Model of Oxygen-Induced Retinopathy.
