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Full Description
This volume provides the latest methods for synthesis, structural analysis, and elucidation of the mechanism. Chapters guide readers through methods on protein cages for nanotechnology, analyze designed protein cages, determine the structures, and even perform theoretical analysis. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.
Authoritative and cutting-edge, Protein Cages: Methods and Protocols aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge.
Contents
Coiled-coil Protein Origami: Design, Isolation and Characterization.- Artificial Protein Cages Assembled Via Gold Coordination.- Reassembly Design of Ferritin Cages.- Protein Cages and Nanostructures Constructed from Protein Nanobuilding Blocks.- Preparation of Cage-like Micellar Assemblies of Engineered Hemoproteins.- Fabrication of Protein Macromolecular Frameworks (PMFs) and Their Application in Catalytic Materials.- Iron Accumulation in Ferritin.- A Generalized Method for Metal Fixation in Horse Spleen L-Ferritin Cage.- Dual Modification of Artificial Protein Cage.- Modification and Production of Encapsulin.- Cryo-electron Microscopy of Protein Cages.- Time-resolved Small-angle X-Ray Scattering of Protein Cage Assembly.- Protein Cage-Stabilized Emulsions: Formulation and Characterization.- A Method to Investigate the Mechanism of Charge Transport across Bio-Molecular Junctions with Ferritin.- Potato Virus X Inactivation and Characterization.- Molecular Dynamics Simulation of Protein Cages.- Coarse-grained Models for Vault Normal Model Analysis.- Evaluating Anti-Tumor Immune Responses of Protein Nanoparticle-Based Cancer Vaccines.- Construction Protocol of Drug-Protein Cage Complexes for Drug Delivery System.- Protein Cage Relaxivity Measurement for Magnetic Resonance Imaging Contrast Agents.- Crystalline Biohybrid Materials Based on Protein Cages.- Production and Purification of Virus-like Particles by Transient Expression in Plants.- Laboratory Scale Production of Complex Proteins Using Charge Complimentary Nanoenvironments.
