Full Description
This second edition provides new and updated methods and protocols for studying tumor angiogenesis in vitro and in vivo. Chapters detail morphological aspects of tumor angiogenesis, aortic ring, ex vivo tissue culture model for anti-angiogenic drug testing, transgenic zebrafish, orthotopic models of ovarian cancer, and uncovering metabolic effects of anti-angiogenic therapy in tumors by induced metabolic bioluminescence imaging.Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and comprehensive, Tumor Angiogenesis Assays: Methods and Protocols, Second Edition is a valuable resource for all researchers interested in learning more about this important and developing field.
Contents
Discovery and development of tumor angiogenesis assays.- The role of the stroma in tumor angiogenesis and progression: the fundamental contribution of Pietro M. Gullino.- Methods for Isolation of Tumor Associated Endothelial Cells for Surface Protein Analysis and Sorting by Flow cytometry.- Perfusion based fluorescent dye labeling to sort cancer cells based on their distance from blood vessels.- Visualizing the interactions that haping the imaging of microenvironment in cancer diseases.- Quantification of tumor and angiogenic-related markers in ovarian cancer models by a digital pathology approach.- Monitoring angiotropic extravascular migratory metastasis in vitro.- Double immunohistochemical staining on formalin-fixed paraffin-embedded tissue samples to study vascular co-option.- Development of Experimental Three-Dimensional Tumor Models to Study Glioblastoma Cancer Stem Cells and Tumor Microenvironment.- IKOSA® CAM Assay application to quantify blood vessels on chick chorioallantoic membrane (CAM).- Application of laser speckle contrast imaging (LSCI) for the angiogenesis measurement of tumors in the chorioallantoic membrane (CAM) model.- The chick CAM as an in vivo system to study stem cell activity.- Detection of possible symmetries in vascular networks by computer assisted image analysis.- Alternative in vivo models to study teratoma.- How to generate a vascular-labeled transgenic zebrafish model to study tumor angiogenesis and extravasation.- 3D human tumor tissues cultured in dynamic conditions as alternative in vitro disease models.
