Full Description
This volume discusses methods used in the fields of molecular and cellular biology for detecting and studying cell death, especially in cancer and cancer therapy. Chapters in this book cover topics such as non-destructive, real-time Annexin V apoptosis assay; assessment of the immune response to tumor cell apoptosis and efferocytosis; mass cytometry assessment of cell phenotypes and signaling states in human whole blood; cell cycle analysis of ER stress and autophagy; and simultaneous detection of inflammasome activation and membrane damage during pyroptosis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and practical, Apoptosis and Cancer: Methods and Protocols is a valuable resource and practical guide for both novice and expert researchers looking into the "meaning of death."
Contents
A Non-Destructive, Real-Time Annexin V Apoptosis Assay.- Cell Death Analysis in Cancer Spheroids from a Microfluidic Device.- Apoptosis Detection by Quantification of Cell Debris in Bright-Field Microscopy Images.- Accurate Enumeration of Apoptotic Cancer Cells Using Flow Cytometry.- Assessment of the Immune Response to Tumor Cell Apoptosis and Efferocytosis.- Quantitative Analysis of Apoptosis and Necrosis in Live Cells Using Flow Cytometry.- A Fluorescence-Microscopic System for Monitoring the Turnover of the Autophagic Substrate p62/SQSTM1.- Single-Cell Profiling of the Intrinsic Apoptotic Pathway by Mass Cytometry (CyTOF).- High-Throughput, Parallel Flow Cytometry Screening of Hundreds of Cell Surface Antigens using Fluorescent Barcoding.- Mass Cytometry Assessment of Cell Phenotypes and Signaling States in Human Whole Blood.- Quantification of Neutrophils Undergoing NET Formation and Distinguishing Mechanisms of Neutrophil Cell Death by Use of a High-Throughput Method.- Profiling of Protein Carbonylations in Ferroptosis by Chemical Proteomics.- Cell Cycle Analysis of ER Stress and Autophagy.- Proximity Ligation In Situ Assay to Monitor Autophagy-Related Protein Interactions and Autophagy in Cancer Cells.- Simultaneous Detection of Inflammasome Activation and Membrane Damage During Pyroptosis.- Cancer and Apoptosis.
