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Full Description
This volume provides essential and fundamental protocols on manipulation chromosome. Chapters details methods on the preparation of mitotic chromosome, chromosome aberration, micronucleus (MN), comet assay, karyotyping, Fluorescent in situ hybridization (FISH), premature chromosome condensation (PCC), immunohistochemistry (IHC) staining, new generation sequencing technology and new chromosome concepts, such as epigenetic and its cause of cancer are presented. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.
Authoritative and cutting-edge, Chromosome Analysis: Methods and Protocols aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge.
Contents
A Basic and Simple Chromosome Preparation Protocol.- Metaphase chromosome preparation and classification of chromosomal aberrations.- Mitotic Index Analysis.- Preparation of Mitotic Cells for Fluorescence Microscopy.- Chemical induced Premature Chromosome Condensation protocol.- Apoptosis Detection Assays.- Alkaline Comet Assay To Detect DNA Damage.- Analysis for Sister Chromatid Exchange.- Cytokinesis Blocked Micronuclei Aberration Analysis.- DNA Damage Foci on Metaphase Chromosomes.- FISH with Whole Chromosome Painting Probes.- Telomere aberration detection by PNA-FISH probe.- Nontraditional Method for Telomere Staining by PNA Probes.- Visualizing Active Replication Regions in S-phase Chromosomes.- Hi-C Analysis to Identify Genome-Wide Chromatin Structural Aberration in Cancer.- CUT&Tag using "stress-free" con A-conjugated Dynabeads®.- Live-cell Synthetic Histone Acetylation by Chemical Catalyst.- Histone Modification Analysis of Low-Mappability Regions.
