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Full Description
This volume provided methods and protocols on recombinant protein production in different plant systems, downstream processing, and strategies to optimize protein expression. Chapters guide readers through recombinant protein production in important plant systems, protein recovery and purification, different strategies to optimise productivity, cloning and fusion protein approaches, and the regulation and freedom to operate analysis of plant-produced proteins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Recombinant Proteins in Plants: Methods and Protocols aims to be useful to newcomers and experienced researchers interested in expanding their expertise in the field of plant-based protein production.
Chapters 6, 8 and 17 are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
Contents
Recombinant Protein Production in Plants - A Brief Overview of Strengths and Challenges.- Production of Recombinant Proteins in Transgenic Tobacco Plants.- Molecular Farming in Seed Crops: Gene Transfer into Barley (Hordeum vulgare) and wheat (Triticum aestivum).- Cell Biology Methods to Study Recombinant Proteins in Seeds.- Production of Recombinant Glycoproteins in Nicotiana tabacum BY-2 Suspension Cells.- Production of Recombinant Proteins by Agrobacterium-mediated Transient Expression.- Specific packaging of custom RNA molecules into Cowpea Mosaic Virus-Like Particles.- Plant-based Cell-Free In Vitro Transcription and Translation of Recombinant Proteins.- Strategies for Efficient and Sustainable Protein Extraction and Purification from Plant Tissues.- Improving Recombinant Protein Recovery from Plant Tissue Using Heat Precipitation.- Techno-economic Modeling and Simulation for Plant-Based Manufacturing of Recombinant Proteins.- Optimization of Vectors and Targeting Strategies Including Goldenbraid and Genome Editing Tools -GoldenBraid assembly of multiplex CRISPR/Cas12a guide RNAs for Gene Editing in Nicotiana benthamiana.- Advanced Fusion Strategies for The Production Of Functionalized Potato Virus X virions.- Knockout of Glycosyltransferases in Nicotiana benthamiana by Genome Editing to Improve Glycosylation of Plant-Produced Proteins.- A bioluminescent Agrobacterium tumefaciens for Imaging Bacterial Metabolic Activity in planta.- Statistical Designs to Improve Downstream Processing.- Regulation of Molecular Farming Products.- Freedom to Operate Analysis of Molecular Farming Projects.
