Full Description
This detailed book serves as a systematic examination of the analytical methods to study the transcription factor NF-κB in physiology and disease. It provides an up-to-date guidebook to navigate both conventional and highly specialized methods to detect and analyze the different signaling pathways of NF-κB activation and contextualize them within organismal physiology and disease pathogenesis, using genetic and biochemical techniques and some of the most advanced computational and systems biology methods. Additionally, the volume includes several examples of approaches utilized by leading experts in the NF-κB field to analyze and modulate NF-κB signaling in specific physiological and disease contexts, along with some of the most promising approaches to pharmacologically target the NF-κB pathway in human disease. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, NF-κB Transcription Factors: Methods and Protocols is an up-to-date guide intended for both basic and translational scientists who are working in the dynamic NF-κB field.
Contents
Measuring NF-kB Phosphorylation and Acetylation.- Biochemical Methods to Analyze the Subcellular Localization of NF- κB Proteins Using Cell Fractionation.- Immunohistochemical Analysis of Expression, Phosphorylation, and Nuclear Translocation of NF-κB Proteins in Human Tissues.- High-Throughput Analysis of the Cell- and DNA Site-Specific Binding of Native NF-κB Dimers Using Nuclear Extract Protein-Binding Microarrays (NextPBMs).- Molecular and Biochemical Approaches to Study the Evolution of NF-κB Signaling in Basal Metazoans.- Methods for Modulating the Pathway of NF-kB Using Short Hairpin RNA (ShRNA).- Immunoblot Analysis of the Regulation of TNF Receptor Family-Induced NF-kB Signaling by c-IAP Proteins.- Methods to Study CARD11-BCL10-MALT1 Dependent Canonical NF-kB Activation in Jurkat T-Cells.- Analysis of Calcium Control of Canonical NF-κB Signaling in B Lymphocytes.- A Kinase Assay for Measuring the Activity of the NIK-IKK1 Complex Induced via the Noncanonical NF-κB Pathway.- Analyze the SUMOylation of IKKγ/NEMO During Genotoxic Stress.- Analysis of the Contribution of NF-kB in the Regulation of Chemotherapy-Induced Cell Senescence by Establishing a Tetracycline-Regulated Cell System.- Methods to Detect NF-κB Activity in Tumor-Associated Macrophage (TAM) Populations.- Methods to Study the Effect of IKK Inhibition on TNF-Inducing Apoptosis and Necroptosis in Cultured Cells.- Use of ChIP-qPCR to Study the Crosstalk between HIF and NF-kB Signaling in Hypoxia and Normoxia.- Methods to Analyze the Roles of TAK1, TRAF6, and NEMO in the Regulation of NF-κB Signaling by RANK Stimulation During Osteoclastogenesis.- NF-kB Signaling in Ex Vivo Mouse Intestinal Organoids.- Extracellular Flux Analysis to Investigate the Impact of NF-κB on Mitochondrial Respiration in Colorectal Carcinoma (CRC).- Conditional Knockout Mouse Models to Study the Roles of Individual NF-κB Transcription Factors in Lymphocytes.- Generation and Surgical Analysis of Genetic Mouse Models to Study NF-κB-Driven Pathogenesis of Diffuse Large B-Cell Lymphoma.- The Screening of Combinatorial Peptide Libraries for Targeting Key Molecules or Protein-Protein Interactions in the NF-κB Pathway.
