Full Description
This volume presents a collection of methods that have contributed to the current understanding of bacterial persisters. Chapters in the book detail general guidelines for measuring persister levels in bacterial cultures, strategies to enrich and resuscitate persister subpopulations, single-cell approaches for visualizing and characterizing persisters, omics techniques and cellular and animal models for studying persistence. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Bacterial Persistence: Methods and Protocols, Second Edition aims to be a useful practical guide to researchers to help further their study in this field.
Contents
Studying Bacterial Persistence: Established Methods And Current Advance.- Antibiotic Tolerance and Persistence Studied Throughout Bacterial Growth Phases.- A Robust Method For Generating, Quantifying, and Testing Large Numbers of Escherichia coli persisters.- Enrichment of Persister Cells through β-lactam-induced Filamentation and Size Separation.- Methods for Enrichment of Bacterial Persister Populations For Phenotypic Screens And Genomic Studies.- Observing Bacterial Persistence at Single-Cell Resolution.- Phenotypic Characterization of Antibiotic Persisters at the Single-Cell Level: From Data Acquisition to Data Analysis.- Microfluidics for Single-Cell Study of Antibiotic Tolerance and Persistence Induced by Nutrient Limitation.- Counting Chromosomes in Individual Bacteria to Quantify their Impacts on Persistence.- Analyzing PERSISTER PROTEOMES with SILAC and Label-Free Methods.- Defining Proteomic Signatures To Predict Multi-Drug Persistence in Pseudomonas aeruginosa.- The use of Experimental Evolution to Study The Response of Pseudomonas aeruginosa to Single or Double Antibiotic Treatment.- Detecting persister awakening determinants.- Monitoring Persister Resuscitation with Flow Cytometry.- Stimulating Aminoglycoside Uptake to Kill Staphylococcus aureus persisters.- In vitro Models for the Study of the Intracellular Activity Of Antibiotics.- Analysis of Salmonella Persister Population Sizes, Dynamics Of Gut Luminal Seeding, And Plasmid Transfer In Mouse Models Of Salmonellosis.- Studying Antibiotic Persistence During Infection.
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