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Full Description
This book provides basic information and different protocols associated with the Entomopathogenic nematodes (EPNs) and their symbiotic bacteria. Entomopathogenic nematodes (EPNs) of the genera Steinernema and Heterorhabditis and their associated bacterial symbionts Xenorhabdus and Photorhabdus aid nematode infective juveniles (IJs) in infecting and killing their insect hosts, creating a unique tripartite complex of host-vector-symbiont interactions. Due to this insect killing capability, EPNs are used as biological control agents of economically important insect pests. They are also a model system to study host-parasite interactions.
It provides a systematic approach to various nematode procedures including pathogenicity, reproduction, foraging behaviour. It gives a brief outline on historical aspects, nematode-bacterium complex, biology and chemical ecology of EPNs. It concisely describes host insect rearing, nematode samplingand storage, isolation techniques, counting, handling and staining of nematodes, characterization including morphological, molecular and ecological studies, mass production, virulence bioassay, field application and efficacy. The book also includes methods and techniques for their associated symbiotic bacteria.
This book serves as a laboratory manual and assists the readers to undertake advanced research in different aspects related to nematodes. It is useful for researchers in the fields of nematology, microbiology, bacteriology and entomology.
Contents
Foreword.- Preface.- Acknowledgement.- Chapter 1: Safety Guidelines.- Chapter 2: Introduction.- Chapter 3: Historical Aspects.- Chapter 4: Nematode-Bacterium Symbiosis.- Chapter 5: Biology.- Chapter 6: Nematode Behaviour.- 7. Chemical Ecology.- Chapter 8: Laboratory Techniques for Entomopathogenic Nematodes.- Chapter 9: Laboratory Techniques for Symbiotic Bacteria.- Chapter 10: Field Application Techniques for Control of Insect Pests.- Chapter 11: Application Efficacy Against Insects in Other than Soil Habitats.- Chapter 12: Recent Advances and Future Prospect.- References.
