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Full Description
This volume explores the latest engineering methods of mammalian cells that are useful for controlling the performance of engineered mammalian cells for future cell-based therapeutics and for better understanding of complex biological systems. The chapters in this book are organized into five parts. Part One described methods to engineer mammalian cells to sense biologically relevant inputs, such as cell contacts and soluble proteins. Part Two looks at techniques to engineer mammalian cells to sense artificial inputs, such as light and ultrasound. Part Three provides cutting-edge CRISPR-Cas-based methods to carry out highly multiplexed genome editing and spatiotemporally controlled genome editing. Part Four discusses ways to control and engineer biological events in mammalian cells in combination with chemical compounds and systems. Part Five explores techniques to engineer specific mammalian cells in targeted manners. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and authoritative, Mammalian Cell Engineering: Methods and Protocols is a valuable resource that allows scientists to successfully carry out their research, thus ultimately contributing to the future advancement of this field.
Contents
Generation of CAR-T Cells by Lentiviral Transduction.- Synthetic Receptors for Sensing Soluble Molecules with Mammalian Cells.- Engineering Mammalian Cells to Control Glucose Homeostasis.- Using Engineered Mammalian Cells for an Epitope-Directed Antibody Affinity Maturation System.- Purification of Specific Cell Populations Differentiated from Stem Cells using MicroRNA-Responsive Synthetic Messenger RNAs.- Green Light-Controlled Gene Switch for Mammalian and Plant Cells.- Sonogenetic Modulation of Cellular Activities in Mammalian Cells.- Constructing Smartphone-Controlled Optogenetic Switches in Mammalian Cells.- Constructing a Smartphone-Controlled Semiautomatic Theranostic System for Glucose Homeostasis in Diabetic Mice.- Construction of Caffeine-Inducible Gene Switches in Mammalian Cells.- Multiplexed Genome Engineering with Cas12a.- Highly Multiplexed Analysis of CRISPR Genome Editing Outcomes in Mammalian Cells.- Optical Control of Genome Editing by Photoactivatable Cas9.- Chemogenetic Control of Protein Localization and Mammalian Cell Signaling by SLIPT.- Engineering Hydrogel Production in Mammalian Cells to Synthetically Mimic RNA Granules.- AgDD System: A Chemical Controllable Protein Aggregates in Cells.- Intracellular Unnatural Catalysis Enabled by an Artificial Metalloenzyme.- Feeder-Free Human Induced Pluripotent Stem Cell Culture using a DNA Aptamer-Based Mimic of Basic Fibroblast Growth Factor.- Protocol for de novo Gene Targeting via In Utero Electroporation.- Magnetically Guided Single Cell Virus Stamping.
