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Full Description
This volume brings together classical and cutting-edge protocols on the spatio-temporal study of the cellular subsets constituting the bone and the marrow in both mouse and human. Chapters details methods on bone marrow (BM) ecosystem, to label, sort, analyse, and culture specific cell subsets as well as techniques allowing the evaluation of the function of some of the cellular elements of the BM. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Bone Marrow Environment: Methods and Protocols aims to help new investigators to pursue the characterization of the BM microenvironment in the coming years.
Contents
Culture, Expansion, and Differentiation of Human Bone Marrow Stromal Cells.- Differentiation and Phenotyping of Murine Osteoclasts from Bone Marrow Progenitors, Monocytes, and Dendritic Cells.- Culture, Expansion, and Differentiation of Mouse Bone-derived Mesenchymal Stromal Cells.- Feeder-Free Differentiation Assay for Mouse Hematopoietic Stem and Progenitor Cells.- In Vitro Analysis of Energy Metabolism in Bone-marrow Mesenchymal Stromal Cells.- Flow Cytometry Analysis of Mouse Hematopoietic Stem and Multipotent Progenitor Cells.- Flow Cytometry-Based Analysis of the Mouse Bone Marrow Stromal and Perivascular Compartment.- Immunophenotyping of the Medullary B cell Compartment in Mouse Models.- Metabolic Analysis of Mouse Haematopoietic Stem and Progenitor Cells.- Multicolor Immunofluorescence Staining Of Paraffin-Embedded Human Bone Marrow Sections.- 3D microscopy of Murine Bone Marrow Hematopoietic Tissues.- Ex Vivo Whole-Mount Imaging of Leukocyte Migration to the Bone Marrow.- Intrafemoral Delivery of Hematopoietic Progenitors.- Imaging of Bone Marrow Plasma Cells and of their Niches.- Intravital Imaging of Bone Marrow Microenvironment in the Mouse Calvaria and Tibia.- Intravital Imaging of Bone Marrow Niches.- Modeling Human Fetal Hematopoiesis in Humanized Mice.- Engraftment of Human Hematopoietic Cells in Biomaterials Implanted In Immunodeficient Mouse Models.- 3D Engineering of Human Hematopoietic Niches in Perfusion Bioreactor.- Manufacturing a Bone Marrow-on-a-Chip Using Maskless Photolithography.- In Vivo Tracking of Hematopoietic Stem and Progenitor Cell Ontogeny by Cellular Barcoding.- Single Cell Analysis of Haematopoietic Stem Cells.
