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Full Description
This volume explores analytical methods to study complex lipid mixtures from plants and algae. The chapters in this book are organized into five parts and cover topics such as basic methods of lipid isolation and analysis; mass spectrometry and NMR analysis; lipid isolation and analysis from plant tissues, cell compartments and organelles; lipid signaling, lipid-protein interactions, and imaging; and lipid databases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and comprehensive, Plant Lipids: Methods and Protocols is a valuable guide for experienced researchers and undergraduate, graduate, and Ph.D. students. This book is also an excellent resource for novice scientists with little to no experience inlipid experiments who are interested in approaching this field experimentally.
Contents
Lipid Isolation from Plants.- Three Methods to Extract Membrane Glycerolipids: Comparing Sensitivity to Lipase Degradation and Yield.- Thin-Layer Chromatography.- Lipid Analysis by Gas Chromatography and Gas Chromatography Mass Spectrometry.- 14C-Tracing of Lipid Metabolism.- Methods of Lipid Analyses for Microalgae: Charophytes, Eustigmatophytes, and Euglenophytes.- Direct Infusion Mass Spectrometry for Complex Lipid Analysis.- Fatty Acid Composition by Total-Acyl-Lipid--Collision Induced Dissociation--Time-of-Flight (TAL-CID-TOF) Mass Spectrometry.- Targeted Analysis of the Plant Lipidome by UPLC-NanoESI-MS/MS.- Mass Spectrometry-Based Profiling of Plant Sphingolipids from Typical and Aberrant Metabolism.- Analysis of Free and Esterified Sterol Content and Composition in Seeds using GC and ESI-MS/MS.- Techniques for the Measurement of Molecular Species of Acyl-CoA in Plants and Microalgae.- Quantification of Acyl-Acyl Carrier Proteins for Fatty Acid Synthesis using LC-MS/MS.- Structural Analysis of Glycosylglycerolipids using NMR Spectroscopy.- Analysis of Extracellular Cell Wall Lipids: Wax, Cutin and Suberin in Leaves, Roots, Fruits, and Seeds.- Isolation of Lipid Droplets for Protein and Lipid Analysis.- Isolation of Plastoglobules for Lipid Analyses.- Isolation of Mitochondria for Lipid Analysis.- Collection and Analysis of Phloem Lipids.- Analyses of Inositol Phosphates and Phosphoinositides by Strong Anion Exchange (SAX)-HPLC.- Analysis of Phosphoinositides from Complex Plant Samples by Solid-Phase Adsorption Chromatography and Subsequent Quantification via Thin-Layer and Gas Chromatography.- Studying Lipid-Protein Interactions Using Protein-Lipid-Overlay and Protein-Liposome-Association Assays.- Investigations of Lipid Binding to Acyl-CoA-Binding Proteins (ACBP) Using Isothermal Titration Calorimetry (ITC).- In Situ Localization of Plant Lipid Metabolites by Matrix Assisted Laser Desorption/Ionization-Mass Spectrometry Imaging (MALDI-MSI).- Plant Lipid Databases.- Lipid Pathway Databases with a Focus on Algae.
