Full Description
This book brings together a collection of methods for the successful detection, culture and investigation of H. pylori, and its associated diseases. Chapters guide the reader through overviews on H. pylori infection, protocols for analyzing human clinical samples, and protocols for characterizing H. pylori, its functional components and its interactions with host cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Helicobacter pylori: Methods and Protocols aims to be a useful practical guide to researchers to help further their study in this field.
Contents
An Overview of Helicobacter pylori Infection.- The Urea Breath Test for the Non-Invasive Detection of Helicobacter pylori.- Real-time Diagnosis of Helicobacter pylori during Endoscopy by Gastric Juice Analysis.- Molecular Detection of Antibiotic-resistant Helicobacter pylori.- Endoscopic Detection of Helicobacter pylori by the Rapid Urease Test.- Culture-based Antimicrobial Susceptibility Testing for Helicobacter pylori.- Purification of Total RNA from Stomach Tissue Biopsies.- Gene Expression Analysis by Reverse Transcription Quantitative PCR.- A Method to Investigate the Helicobacter pylori-associated DNA Methylome.- Profiling the Gastrointestinal Microbiota.- Helicobacter pylori Virulence Factor Genotyping.- Generation of Isogenic Mutant Strains of Helicobacter pylori.- Isolation of Outer Membrane Vesicles from Helicobacter pylori.- AMouse Model of Helicobacter pylori Infection.- Investigating the Interaction of Helicobacter pylori with the Gastric Mucosa.- Evaluating Host Responses to Helicobacter pylori using ELISA and Western Blot.- Analysis of Innate Immune Responses to Helicobacter pylori.- Analysis of T Cell Responses to Helicobacter pylori Infection.- Miniaturised In Vitro Assays to Study Cellular Phenotypic Characteristics: Proliferation, Migration, Invasion, and Anoikis-resistance.
