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Full Description
This volume looks at in vitro disease models representing the respiratory, hepatobiliary, osteochondral, nervous, dermal, ocular, immune system, and pathological biological processes like tumorigenesis for stem cell research. The chapters in this book cover a range of diseases and application of various stem cells such as adult stem cells and iPS. Chapters also discuss new methods to characterize and manipulate stem cells with the aim to better understand and improve their biological performance. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and thorough, In Vitro Models for Stem Cell Therapy: Methods and Protocols is a valuable resource for researchers and scientists interested in learning more about this exciting field
Contents
Consistent Inclusion of Mesenchymal Stem Cells into In Vitro Tumor Models.- Pluripotent Stem Cells for Cell Therapy.- In Vitro Methods for the Study of Glioblastoma Stem-Like Cell Radiosensitivity.- Bioimaging of Mesenchymal Stem Cells Spatial Distribution and Interactions with 3D In Vitro Tumor Spheroids.- Investigation of the MSC Paracrine Effects on Alveolar-Capillary Barrier Integrity in the In Vitro Models of ARDS.- In Vitro Methods to Evaluate the Effects of Mesenchymal Stem Cells on TGFβ1-induced Pulmonary Fibrosis.- Study of Mesenchymal Stem Cell-Mediated Mitochondrial Transfer in In Vitro Models of Oxidant-Mediated Airway Epithelial and Smooth Muscle Cell Injury.- Co-Culture of Peripheral Blood Mononuclear Cells and Endothelial Colony Forming Cells from Cord Blood of Preterm Born Babies.- Ex Vivo Model of Spontaneous Neuroretinal Degeneration for Evaluating Stem Cells' Paracrine Properties.- Ex Vivo Normothermic Hypoxic Rat Liver Perfusion Model: An Experimental Setting for Organ Recondition and Pharmacological Intervention.- Co-Culture of Human Mesenchymal Stromal Cells and Primary Mouse Hepatocytes.- A 3D Dynamic In Vitro Model of Inflammatory Tendon Disease.- Generation of Epidermal Equivalents from Hair Follicle Melanocytes, Keratinocytes, and Dermal Fibroblasts.- Using Gene Expression Music Algorithms (GEMusicA) for the Characterization of Human Stem Cells.- Evaluation of Extracellular Vesicles from Adipose Tissue-Derived Mesenchymal Stem Cells in Primary Human Chondrocytes from Patients with Osteoarthritis.- Generation of Neural Stem Cells from Pluripotent Stem Cells for Characterization of Early Neuronal Development.- Engineered Tissues Made from Human iPSC-derived Schwann Cells for Investigating Peripheral Nerve Regeneration In Vitro.- Modulation of a Stem Cell Gene: LGR4 Knockout in a Human Cell Line by CRISPR/Cas Method.- In Vitro Tool: 3D Cell Culture of Human Adipose Tissue-Derived Mesenchymal Stromal Cells on Low Stiffness Silicone Scaffolds.
