Full Description
This volume discusses the latest protocols used by scientists to understand ZIKV and how to mitigate its presence. These studies will benefit in the detection, diagnosis, management, treatment, and prevention of this infection. The chapters in this book cover techniques in virus isolation, purification, and ways to titrate the virus; recent advancements made in the development of modified molecular techniques; and a discussion on Zika virus itself to help readers better understand this pathogen. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and practical, Zika Virus: Methods and Protocols is a valuable resource for both novice and expert scientists interested in learning more about this important topic.
Contents
Zika Virus: A Brief History and Review of its Pathogenesis Rediscovered.- Zika Virus Isolation, Purification, and Titration.- Evaluating Zika Virus Pathogenesis in Immunocompromised Mice.- Droplet Digital PCR and Immunohistochemistry Techniques to Detect Zika Virus in the Central Nervous System of Mice.- A Plaque Reduction Neutralization Test for the Detection of ZIKV-Specific Antibodies.- Analysis of Serum Anti-Zika Virus Antibodies by Focus Reduction Neutralization Test.- Enzyme-Linked Immunosorbent Assay and Quantitative Reverse Transcription PCR as a Technique to Analyze Inflammation.- Production and Purification of Zika Virus NS1 Glycoprotein in HEK293 Cells.- Optimization of Small-Scale Production of Zika Virus Envelope Glycoprotein by Transient Expression in HEK293 Cells for ELISA.- Zika Virus Peptide ELISA (ZIKV-NS2B-concat ELISA) for Detection of IgG Antibodies to Zika Virus Infection.- Zika Virus Amplification using Strand Displacement Isothermal Method and Sequencing using Nanopore Technology.- Detection of Zika Virus using Reverse Transcription-Loop-Mediated Isothermal Amplification (RT-LAMP).- Development and Validation of Real-Time RT-LAMP Assays for the Specific Detection of Zika Virus.- Comparative Analysis of Zika Virus Detection by RT-qPCR, RT-LAMP, and RT-RPA.- A Porcine Model of Zika Virus Infection to Profile the In Utero Interferon Alpha Response.- Isolation and Detection of Zika Virus-Infected Rhesus Macaques Lymph Node Cells and Splenocytes.- Discovery of Zika Virus Dependency and Restriction Factors using Flow-Based Arrayed CRISPR Screening for Identification of Targets (FACS-IT).- Zika Virus Targets Multiple Tissues and Cell Types During the First Trimester of Pregnancy.- Use of Primary Human Fetal Astrocytes and Tissue Explants as Ex Vivo Models to Study Zika Virus Infection of the Developing Brain.- Isolation of Monoclonal Antibodies from ZikaVirus-Infected Patient Samples.
