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Full Description
This volume details methods used to track cells in the body and will serve as a reference for preclinical and clinical researchers in the fields of medicine and biomedical science. Chapters guide readers through protocols on bioluminescence imaging, fluorescence imaging, magnetic resonance imaging (MRI), ultrasound, computed tomography, and positron emission spectroscopy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Cell Tracking: Methods and Protocols aims to ensure successful results in the further study of this vital field.
Contents
Using Bioengineered Bioluminescence to Track Stem Cell Transplantation In Vivo.- Using Naturally Occurring Bioluminescent Enzymes to Track Specific Cell Populations.- In vivo Epithelial Metabolic Imaging using a Topical Fluorescent Glucose Analog.- Lipophilic Near Infrared Dyes for in vivo Fluorescent Cell Tracking.- Energy-transfer Systems for in vivo Tracking.- Using Bioengineered Fluorescence for Selective in vivo and ex vivo Tracking of Intestinal Organoids Derived from Human Pluripotent Stem Cells.- Using iRFP Genetic Labeling Technology to Track Tumorogenesis of Transplanted CRISPR/Cas9-edited iPSC in Skeletal Muscle.- Upconversion Fluorescent Nanoprobe for Highly-sensitive in vivo Cell Tracking.- Magnetic Resonance Imaging of Single Cells.- MRI Tracking of Dendritic Cells Loaded with Superparamagnetic Iron Oxide Nanoparticles.- Protease-activated Sensors for InVivo Imaging of Cell Populations.- Antibody Targeted Magnetic Nanoparticles to Track Immune Cells in Vivo.- Stem Cell Tracking with Nanoparticle-Based Ultrasound Contrast Agents.- Gold Nanoparticles as Computer Tomography Marker for Stem Cell Tracking.- Non-Invasive Tracking of Implanted Cells: Superparamagnetic Iron Oxide Nanoparticles as a Long-Term, Multi-Modal Imaging Label.- Comparison of the Efficacy and Sensitivity of Alternative PET Reporter Gene/PET Reporter Probe Systems that Minimizes Biological Variables.
