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Full Description
This volume provides current and new advanced methods and protocols to study T cells. Chapters guide readers through T cell diversity using mass cytometry, analyzing T cells from single cell level, CRISPR/Cas9 techniques to study the T cell activation, techniques to study subsets of Tcell's, procedures to study artificial antigen presentosomes for T cell activation, techniques to study the T cell development, two-photon microscopy, and MAIT cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, T-Cell Receptor Signaling: Methods and Protocols aims to provide a wide range of approaches and be an invaluable resource for present and future generations of T cell researchers.
Contents
Exploration of T Cell Diversity using Mass Cytometry.- Analysis of Transcriptional Profiling of Immune Cells at the Single-cell Level.- Simultaneous Measurement of Surface Proteins and Gene Expression from Single Cells.- A Carrier Strategy for Mass Cytometry Analysis of Small Numbers of Cells.- CRISPR/Cas9-based Genetic Screening to Study T Cell Function.- Preferential Expansion of CD4+Foxp3+ Regulatory T Cells (Tregs) In Vitro by Tumor Necrosis Factor.- CD4+ T Cell Differentiation in vitro.- Characterization of Immune Cell Subset Expansion in Response to Therapeutic Treatment in Mice.- Primary T Cell Transduction to Study Follicular Helper T Cell Differentiation.- In vitro Generation of Stem Cell Memory-like T Cells from Activated T Cells.- Artificial Antigen Presentosomes for T Cell Activation.- Imaging Chimeric Antigen Receptor (CAR) -triggered T Cell Activation.- Assessing the Impact of Phytochemicals on Immune Checkpoints: Implications for Cancer Immunotherapy.- Testing the Efficiency and Kinetics of Negative Selection Using Thymic Slices.- Investigating T Cell Receptor Signaling Dynamics in situ by Two-photon Microscopy of Thymocytes Expressing Genetic Reporters in Low-Density Chimeras.- Retroviral Gene Transduction into T-cell Progenitors for Analysis of T Cell Development in the Thymus.- An Integrated Strategy for Identifying Targets of Ubiquitin-mediated Degradation in CD4+ T Cells.- Radioisotope Based Protocol for Determination of Central Carbon Metabolism in T Cells.- Assessment of Immune Protective T cell Repertoire in Humans Immunized with Novel Tuberculosis Vaccines.- Studying Peripheral T Cell Homeostasis in Mice; A Concise Technical Review.- Detection, Expansion, and Isolation of Human MAIT Cells.- In vitro Differentiation of CD4+ T Cell Effector and Regulatory Subsets.
