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Full Description
The field of Parasitology has seen tremendous progress in the last 2-3 decades with the advent of molecular biology and its application to the study of parasites. This has opened new vistas in the understanding of the taxonomy, pathogenesis, immunological mechanisms of many parasites, along with designing of improved diagnostic tests and application of molecular epidemiology in tracking and controlling these diseases. Parija's Immunologic Diagnostics in Parasitology: Principles Techniques and Applications is an attempt to cover the existing knowledge along with the newer findings so that up-to-date information can be condensed in one volume.
The book has been divided into three parts. Part 1 deals with the background information on immunological techniques including history, principles, sample collection and specimen processing, and quality assurance of these tests. Part II covers detailed discussion about all the important existing immunological test formats along with step-by-step description for development of experimental or in-house tests. Part III includes chapters on some of the recent tests which are gaining importance like point-of-care tests and immuno-PCR. There are four annexures which are on designing of a serological laboratory, commercial suppliers of immunodiagnostic kits for parasitic diseases, and the list of suppliers for biologicals, chemicals, and labwares.
Parija's Immunologic Diagnostics in Parasitology: Principles Techniques and Applications is an effort to inform and engage a wide spectrum of audience, which include postgraduate or doctoral students and faculty members across the disciplines of Medical and, Veterinary sciences. The present volume is intended both as a textbook and a procedure handbook.
Contents
PART I: Principles of Immunodiagnostics in Parasitic Infections
Chapter 1: Introduction to Parasitology. Chapter 2: Immunodiagnostics of Parasitic Infections: A Chronological History. Chapter 3: Immunological Responses in Parasitic infections. Chapter 4: Principles of Immunological Techniques. Chapter 5: Collection, Transport and Preservation of specimens. Chapter 6: Quality Assurance in Immunological Tests.
PART II: Immunodiagnostics in Parasitic Infections
UNIT 1: ANTIGEN AND ANTIBODY BASED SEROLOGICAL TESTS
Chapter 7: Carrier Particles Based Agglutination Tests. Chapter 8: Precipitation Reaction Based Tests. Chapter 9: Complement Fixation Based Tests. Chapter 10: Enzyme Based Tests. Chapter 11: Fluorescent Dye Based Tests. Chapter 12: Immunoblot Tests. Chapter 13: Chemiluminescence Based Tests.
UNIT 2: MISCELLANEOUS TESTS
Chapter 14: Immunohistochemical Techniques. Chapter 15: Intradermal Skin Tests, Dye Test. Chapter 16: Immunodiagnostics in Animals of Zoonotic Importance.
PART III: RECENT TRENDS IN IMMUNODIAGNOSTICS OF PARASITIC INFECTIONS
Chapter 17: Rapid Point of Care Tests. Chapter 18: Biomarkers and Evolving Molecular Immunodiagnostic Tests (PCR-ELISA, Flow Cytometry). Chapter 19: Immunoelectron Microscopy.
ANNEXURES
I. Designing of an Immunodiagnostic Laboratory. II. Diagnostic Commercial Immunological Kits for Parasitic Infections. III. Commercial Sources of Reagents and Chemicals. IV. Indian Academy of Tropical Parasitology (IATP) Guidelines for Parasitic Disease Diagnosis.
