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Full Description
This outstanding text focuses on providing professionals and students working in the pharmaceutical and biotechnology field with the background necessary for developing of a product or process and with the necessary rigor required by federal regulatory agencies in the pharmaceutical industry. The material will enable teachers, lecturers and professors in biotechnology to prepare courses on basic concepts and applications for the purification of biotechnological products of industrial interest. These can be applied in practice, for example, with projects on purification development on an industrial scale or useful unit operations for the development of bioproducts of commercial interest.
Features:
Purification and development of new bioproducts and improvement of those being produced
Provides a background and concepts on the purification of biomolecules and with an industrial perspective
It allows professionals to understand the entire process of developing a biopharmaceutical or bio-food, from bench to industry in biotechnology; one of the fastest-growing sectors of the economy
It promotes the dissemination of information in a didactic way which is of paramount importance for interdisciplinary fields
It enables the reader to follow step-by-step stages of the development of a new biopharmaceutical, and allows the optimization of existing processes
Contents
Chapter 1 Introduction
Chapter 2 Purification processes: analytical methods and enzyme stability
Chapter 3 Cell Disruption
Chapter 4 Filtration and Centrifugation
Chapter 5 Membrane Separation Processes
Chapter 6 Protein Precipitation
Chapter 7 Liquid-liquid extraction in aqueous two-phase systems
Chapter 8 Introduction to chromatography
Chapter 9 Size exclusion chromatography
Chapter 10 Iion Exchange chromatography
Chapter 11 Hydrophobic interaction chromatography
Chapter 12 Affinity Chromatography
Chapter 13 Adsorption chromatography in monolithic columns
Chapter 14 Chromatography: expanded bed adsorption (EBA)
Chapter 15 Continuous Chromatography using a Simulated Moving Bed System
Chapter 16 Chromatography scale-up
Chapter 17 Crystallization
Chapter 18 Distillation
Chapter 19 Plasmid Purification
Chapter 20 Purification of monoclonal antibodies
Chapter 21 Peptides of biotechnological interest: general concepts, production, purification and chemical characterization
Chapter 22 Integration of steps in the production of biotechnological products
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