- ホーム
- > 洋書
- > 英文書
- > Science / Mathematics
Full Description
This book discusses recent advancements and updates in the molecular diagnostics of fungal phytopathogens. The techniques covered in the seventeen chapters help in understanding the importance of early detection of fungal pathogens and their toxins in both post- and pre-harvest stages of crop production. Molecular Approaches for the Detection of Fungal Phytopathogens describes the fungal identification and isolation from different sources such as food, feed and cereals using molecular approaches. These include variants of polymerase chain reaction (PCR) involving: generic (genus and species) and gene-specific primers, RNA-sequence-based next-generation sequences, microarrays, serial analysis of gene expression, fluorescence in-situ hybridization, loop-mediated isothermal amplification, macroarrays, PCR-denaturing gradient gel electrophoresis, magnetic capture hybridization-PCR, nanotechnology, and immunoassays providing faster and more sensitive approaches than traditional methods that overcome challenges by developing novel disease control strategies.
Features
Explores various diagnostic strategies for phytopathogens
Discusses early detection methods when fungal spores are low or there are no visible symptoms
Provides a historical perspective and compares the traditional methods of detection with the current molecular techniques
Describes details of the protocol of every technique mentioned
Covers point-of-care testing for fungal pathogens and quality assurance for molecular testing in plant health
Contents
1: Fungal phytopathogens and importance of their early detection through conventional methods. 2: Fungal phytopathogens diagnostic: A historical perspective. 3: Methodology for the Isolation and Identification of Fungal Pathogens. 4: Polymerase chain reaction and its variants as detection tool for fungal phytopathogens. 5: Loop-mediated isothermal amplification (LAMP) for the detection and quantification of fungal phytopathogens. 6: Oligonucleotide array approaches for the detection of plant pathogenic fungi. 7: An overview on application and advancements of lateral flow assay technique in effective detection of fungal phytopathogens. 8: DNA Barcoding for Identification of Fungal Pathogens. 9: Next Generation Sequencing, a new opportunity for discovering fungal phytopathogens. 10: In-situ hybridization for the detection of fungal phytopathogens. 11: Serological assays for the detection of phytopathogenic fungi. 12: Detection of fungal phytopathogens by nanotechnology method. 13: Aptamers: A Promising Tool for the Detection of Phytopathogenic Fungal Toxins. 14: On-site diagnostic techniques for fungal pathogens. 15: Policies, Regulations and Quality Assurance Requirements for Sustainable Agriculture. 16: Policies, Regulations and Quality Assurance requirements for the agri-food industry. Chapter 17: Further Directions in Fungal Phytopathogen Diagnostics.
