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Full Description
DNA and RNA extraction methods from a variety of tissues and samples are now routine, including extraction from single cells. Many methods are now automated. Sequencing efficiency has reached the point where it is now possible to obtain gigabases of data, both quickly and inexpensively. Such methods permit the identification of gene versions, including those associated with disease (e.g. small nucleotide polymorphism analyses, or SNPs). The general public as well as clinicians can now access a wide variety of literature on the molecular bases of diseases, allowing them to better assess disease risks and treatments. This volume concentrates on medically-focused methods, and therefore the major audience will be medical professionals, students, and those involved in medically-related research endeavors. There are also papers in this volume dealing specifically with methods developed to analyze large sequence data sets. Many methods reviewed herein are more broadly applicable to other fields in biology, chemistry, bioinformatics, and bioengineering, and are intended for a broad readership.
Key Features
Summarizes nucleic acid extractions from a wide variety of tissues and cells
Describes processes of nucleic acid preservation
Reviews forensic sampling, detection of nucleic acids, and delivery of nucleic acids to multicellular organisms
Provides essential guidance for sequencing, sequence analysis, database searches, and phylogenetic analyses
Includes additional methods useful for analysis of nucleic acids and proteins
Related Titles
DeSalle, et al. Phylogenomics: A Primer (ISBN 978-0-3670-2849-7).
Jennings, W. B. Phylogenomic Data Acquisition: Principles and Practice (ISBN 978-0-3678-6980-9).
Wang, X. Next-Generation Sequencing Data Analysis (ISBN 978-1-4822-1788-9)
Sung, W.-K. Algorithms for Next-Generation Sequencing (ISBN 978-0-3676-5797-0)
Contents
Preface. List of Contributors. Nucleic Acid Extraction from Diverse Samples. DNA Extraction from Mummified Tissues. Commercial DNA Extraction Kits. Automated Nucleic Acid Extraction. Forensic DNA Samples: Collection and Handling. DNA Preservation. RNA Storage. Disposable Electrochemical DNA Biosensors. Handheld Nucleic Acid Analyzer. DNA-Binding Fluorophores. Fluorescence Resonance Energy Transfer (FRET). LightUp® Probes. Invader® Assay. Laser-Capture Microdissection. Helper-Dependent Adenoviral Vectors. Retroviral Vectors. Liposomal Nonviral Delivery Vehicles. Polymer-Based Nonviral Nucleic Acid Delivery and Genome Editing. Automated DNA Sequencing. DNA Sequencing Methods. Differential Sequencing by Mass Spectrometry. Brief Guide to Conducting Biological Database Searches. Accessing Genomic Databases. Phylogenetics, Comparative Genomics, and Phylogenomics. DNannotator: Annotation Software Tool Kit for Regional Genomic Sequences. ESTAnnotator: A Tool for High-Throughput EST Annotation. The Basics of Omics. Ribotyping. Forensic DNA Typing: Y Chromosome. Forensic Identification: An Overview on Molecular Diagnostic Technology. Denaturing Gradient Gel Electrophoresis (DGGE). Heteroduplex Analysis (HA). NEBcutter: A Program to Cleave DNA with Restriction Enzymes. Differential Display (DD) Analysis. Protein Truncation Test (PTT). Index.
