炎症プロトコール<br>Inflammation Protocols (Methods in Molecular Biology, V. 225)

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炎症プロトコール
Inflammation Protocols (Methods in Molecular Biology, V. 225)

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  • 製本 Hardcover:ハードカバー版/ページ数 378 p.
  • 言語 ENG
  • 商品コード 9780896039704
  • DDC分類 616.0473

基本説明

Sections: (1) in vitro systems for studying inflammation, (2) in vivo models, and (3) relevant pharmacodynamic measurements for assessing anti-inflammatory compounds.

Full Description


Inflammation has been described as the basis of many pathologies of human disease. When one considers the updated signs of inflammation, they would be vasodilation, cell migration, and, in the case of chronic inflam- tion, cell proliferation, often with an underlying autoimmune basis. Gen- ally, inflammation may be divided into acute, chronic, and autoimmune, - though the editors believe that most, if not all, chronic states are often the result of an autoimmune response to an endogenous antigen. Thus, a proper understanding of the inflammatory basis may provide clues to new therap- tic targets not only in classical inflammatory diseases, but atherosclerosis, cancer, and ischemic heart disease as well. The lack of advances in classical inflammatory diseases, such as rh- matoid arthritis, may in part arise from a failure to classify the disease into different forms. That different forms exist is exemplified in patients with d- fering responses to existing antiinflammatory drugs, ranging from nonresponders to very positive responders for a particular nonsteroidal an- inflammatory drug (NSAID). Though researchers have progressively unr- eled the mechanisms, the story is far from complete. It should also be noted that the inflammatory response is part of the innate immune response, or to use John Hunter's words in 1795, "inflammation is a salutary response." That may be applied in particular to the defensive response to invading micro- ganisms.

Table of Contents

Preface                                            v
Contributors xi
Part I. In Vitro Systems for Studying Aspects
of the Inflammatory Response and Testing
Antiinflammatory Drugs
Key Stages in the Acute Inflammatory 3 (4)
Response and Their Relevance as Therapeutic
Targets: Introduction to Part 1
Paul G. Winyard
IκB Kinase and NF-κB Signaling 7 (12)
in Response to Pro-Inflammatory Cytokines
Mireille Delhase
Screening for Inhibitors of Transcription 19 (6)
Factors Using Luciferase Reporter Gene
Expression in Transfected Cells
Deborah Phippard
Anthony M. Manning
Adhesion Molecule Expression on 25 (10)
Cytokine-Stimulated Human Endothelial Cells
Susan L. Cuvelier
Kamala D. Patel
Phagocytosis by Inflammatory Phagocytes: 35 (12)
Experimental Strategies for Stimulation and
Quantification
M. Rachel Morris
Sharon Dewitt
Iraj Laffafian
Maurice B. Hallett
Cytosolic Ca2+ Measurement and Imaging in 47 (14)
Inflammatory Cells
Sharon Dewitt
Iraj Laffafian
M. Rachel Morris
Maurice B. Hallett
Detection and Visualization of Oxidase 61 (8)
Activity in Phagocytes
Maurice B. Hallett
Caroline Cole
Sharon Dewitt
Measurement of Complement Activation 69 (8)
Tom Eirik Mollnes
Measurement of Matrix Metalloproteinase 77 (12)
Activities in the Medium of Cultured
Synoviocytes Using Zymography
Linda Troeberg
Hideaki Nagase
Measurement of Aggrecanase-Generated 89 (10)
Interglobular Domain Catabolites in the
Medium and Extracts of Cartilage Explants
Using Western Blot Analysis
Clare E. Hughes
Christopher B. Little
Bruce Caterson
In Vitro Model of Human Articular Cartilage 99 (10)
Degradation
William D. Shingleton
Part II. In Vivo Models of Inflammation
In Vivo Models of Inflammation: 109(6)
Introduction to Part 2
Derek A. Willoughby
Carrageenan-Induced Paw Edema in the Rat 115(8)
and Mouse
Christopher J. Morris
Pleural Models of Inflammation: Immune and 123(6)
Nonimmune
Adrian R. Moore
Models of Acute Inflammation in the Ear 129(10)
Miklos Gabor
Migration of Specific Leukocyte Subsets in 139(8)
Response to Cytokine or Chemokine
Application In Vivo
Mauro Perretti
Stephen J. Getting
Inflammatory Joint Disease: Clinical, 147(14)
Histological, and Molecular Parameters of
Acute and Chronic Inflammation and Tissue
Destruction
Nancy L. McCartney-Francis
James Chan
Sharon M. Wahl
The Assessment of Inflammation, Cartilage 161(14)
Matrix, and Bone Loss in Experimental
Monoarticular Arthritis of the Rat
Michael P. Seed
Collagen-Induced Arthritis 175(6)
Adrian R. Moore
Air-Pouch Models of Inflammation and 181(10)
Modifications for the Study of
Granuloma-Mediated Cartilage Degradation
Paul Colville-Nash
Toby Lawrence
Quantitative Analysis of Angiogenesis Using 191(8)
the Murine Chronic Granulomatous Air Pouch
Chandan A. S. Alam
Models of Coronary Artery Occlusion and 199(10)
Reperfusion for the Discovery of Novel
Antiischemic and Antiinflammatory Drugs for
the Heart
Nicole S. Wayman
Michelle C. McDonald
Prabal K. Chatterjee
Christoph Thiemermann
Assessment of Anticolitic Drugs in the 209(14)
Trinitrobenzene Sulfonic Acid (TNBS) Rat
Model of Inflammatory Bowel Disease
Brendan J. R. Whittle
Maryan Cavicchi
Dominique Lamarque
An In Vivo Model of Ischemia/Reperfusion 223(16)
and Inflammation of the Kidneys of the Rat
Prabal K. Chatterjee
Christoph Thiemermann
In Vivo Models of Inflammation: Immune 239(10)
Rejection and Skin Transplantation In Vivo
Isabelle Binet
Kathryn J. Wood
Wound Healing: A Model of Dermal Wound 249(14)
Repair
Annette Tomlinson
Mark W. J. Ferguson
Part III. Pharmacodynamic Endpoints in
Experimental Models and in Clinical Studies
in Humans
An Iconoclastic Approach to 263(6)
Pharmacodynamics in Model Systems: Their
Relevance to Humans: Introduction to Part 3
David R. Blake
Gordon J. Taylor
A Reply to ``An Iconoclastic Approach to 269(4)
Pharmacodynamics in Model Systems: Their
Relevance to Humans''
Derek A. Willoughby
Quantifying Inflammation In Vivo Using 273(10)
Radiolabeled Antibodies and Leukocytes
Diane Marshall
Dorian O. Haskard
Immunoperoxidase Histochemistry for the 283(8)
Detection of Cellular Adhesion Molecule,
Cytokine, and Chemokine Expression in the
Arthritic Synovium
Zoltan Szekanecz
Alisa E. Koch
Roles of Nitric Oxide and Superoxide in 291(14)
Inflammation
Daniela Salvemini
Harry Ischiropoulos
Salvatore Cuzzocrea
Analysis of Nitrite and Nitrate in the 305(16)
Study of Inflammation
Claire A. Davies
Sophie A. Rocks
Meg C. O'Shaughnessy
David Perrett
Paul G. Winyard
In Vivo Assays for COX-2 321(8)
Chi-Chung Chan
Measurement of 8-epi-PGF2α as a 329(14)
Marker of Lipid Peroxidation In Vivo by
Immunoaffinity Extraction and Gas
Chromatography-Mass Spectrometry
Nitin K. Gopaul
Erik E. Anggard
Laboratory Assessment of the Acute Phase 343(10)
Response: Using CRP as a Model
Robert F. Ritchie
Thomas B. Ledue
Assays of Matrix Metalloproteinases (MMPs) 353(12)
and MMP Inhibitors: Bioassays and
Immunoassays Applicable to Cell Culture
Medium, Serum, and Synovial Fluid
Jon B. Catterall
Tim E. Cawston
Index 365