Lab Ref : A Handbook of Recipes, Reagents, and Other Reference Tools for Use at the Bench 〈2〉 （1 SPI）

Lab Ref : A Handbook of Recipes, Reagents, and Other Reference Tools for Use at the Bench 〈2〉（1 SPI）

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Lab Ref : A Handbook of Recipes, Reagents, and Other Reference Tools for Use at the Bench 〈2〉（1 SPI）

Mellick, Albert S. (EDT)/Rodgers, Linda (EDT)

Cold Spring Harbor Laboratory Pr（2006/12発売）

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製本 Hardcover:ハードカバー版／ページ数 248 p.
言語 ENG
商品コード 9780879698157
DDC分類 572.078

Full Description

Like its predecessor Lab Ref, Volume 1, this book is a handy benchtop source of recipes and information needed for common laboratory protocols. Lab Ref, Volume 2 contains recipes for procedures as diverse as RNA interference, imaging, proteomics, and quantitative nucleic acid analysis, and is organized by application for quick and easy reference. All recipes were derived from recent manuals published by Cold Spring Harbor Laboratory Press, and each recipe is cross-referenced to its manual of origin. Reference tables and charts are included. To meet the growing need for computational resources in the biology laboratory, Lab Ref, Volume 2 also has an expanded Web site section with sources for biological materials, databases for acquiring and annotating genomic and proteomic data, and links to downloadable software.

Introduction SECTION 1: QUANTITATIVE NUCLEIC ACID ANALYSIS Isolation of Total DNA Isolation of Total RNA Selection of Poly(A) RNA from Total Isolation and Amplification of RNA from Single Cells and Small Amounts of Tissue Hybridization Analysis Preparation of Nucleic Acid (Target) for Array Hybridization Analysis Preparation of Nucleic Acid (Probe) for Array Hybridization Analysis Other Quantitative Methods SECTION 2: RNAi RNAi in C. elegans RNAi in D. melanogaster RNAi in Plants RNAi in Mammalians RNAi in Mouse Oocytes and Early Embryos SECTION 3: IMAGING Preparation of Embryos for Live Cell Imaging Imaging Fixed Tissue Preparation of Mice for Imaging Intracellular Imaging General Information for Imaging SECTION 4: PROTEIN ANALYSIS/PROTEOMICS Basic Methods in Protein Analysis for Proteomics Extraction of Proteins Advanced Protocols in Identifying Protein-Protein Interactions for Proteomics SECTION 5: ARABIDOPSIS Microscopy Genetic Manipulation Protein Extraction Nuclear Extraction Histochemistry of GUS Fusion SECTION 6: MURINE In Vitro Culturing of Embryonic Stem Cells Genotyping Immunohistochemistry of Whole-mount Embryos Other Buffers and Solutions SECTION 7: STORAGE AND SHIPMENT OF BIOLOGICAL SAMPLES SECTION 8: USEFUL FACTS AND TABLES A. Useful Information and Reference Tables B. Nomenclature Guide C. Useful World Wide Web Sites Appendix Index