基本説明
Covers topics ranging from a discussion of the methodologies utilized in structural virology to the role of virus capsid structures in determining the host cell infection and therapeutic strategies, including the design of antiviral drugs and development of viral capsids as vehicles for foreign gene delivery.
Full Description
Over the last ten years, much effort has been devoted to improving the biophysical techniques used in the study of viruses. This has resulted in the visualization of these large macromolecular assemblages at atomic level, thus providing the platform for functional interpretation and therapeutic design. Structural Virology covers a wide range of topics and is split into three sections. The first discusses the vast biophysical methodologies used in structural virology, including sample production and purification, confocal microscopy, mass spectrometry, negative-stain and cryo-electron microscopy, X-ray crystallography and nuclear magnetic resonance spectroscopy. The second discusses the role of virus capsid protein structures in determining the functional roles required for receptor recognition, cellular entry, capsid assembly, genome packaging and mechanisms of host immune system evasion. The last section discusses therapeutic strategies based on virus protein structures, including the design of antiviral drugs and the development of viral capsids as vehicles for foreign gene delivery. Each topic covered will begin with a review of the current literature followed by a more detailed discussion of experimental procedures, a step in the viral life cycle, or strategies for therapeutic development. With contributions from experts in the field of structural biology and virology this exceptional monograph will appeal to biomedical scientists involved in basic and /or applied research on viruses. It also provides up-to-date reference material for students entering the field of structural virology as well as scientists already familiar with the area.
Contents
Introduction: Principles of Virus Structure;Section 1Methodologies for Structural Analysis of Viruses;
Production and Purification of Viruses for Structural Studies;
Microscopic (Confocal) Analysis of Viral Entry and Infection;
Cryo-electron Microscopy of Virus Infection - Tomography and Asymmetric Structure Determination;
Cryo-electron Microscopy of Viruses - 3D of Virus Capsids;
X-ray Crystallography of Virus Capsids;
Structural Studies of Viral Proteins;
Probing Viral Capsid Structures in Solution;
Section 2Structure to Function Correlation for Viruses;
Evolution of Viral Capsid Structures - the Three Domains of Life;
Mechanisms of Virus Capsid Assembly;
Mechanisms of Genome Packaging;
Attachment and Viral entry - Receptor Recognition in Viral Pathogenesis;
Attachment and Entry - Viral Cell Fusion;
Virus Antibody Recognition;
Section 3Therapeutic Strategies Based on Viral Structures;
Development of anti-HIV drugs;
Design of Influenza Vaccines and Antiviral Agents;
Engineering Viral Capsids as Nano Tools;
Viral Vectors for Gene Delivery;
