代謝工学ハンドブック（全２巻） The Metabolic Pathway Engineering Handbook (2-Volume Set) （1 SLP）

Smolke, Christina D. (EDT)

CRC Pr I Llc（2009/08発売）

• 製本 Hardcover:ハードカバー版／ページ数 678 p.
• 言語 ENG
• 商品コード 9780849339233
• DDC分類 660

基本説明

代謝工学に関する最新研究成果を集約した便覧。各巻分売可。
Vol. 1: Fundamentals provides an overview of metabolic pathway engineering with a look towards the future. It discusses cellular metabolism, including transport processes inside the cell and energy generating reactions, as well as rare metabolic conversions. Vol.2: Tool and Applications delves into evolutionary tools as well as. gene expression tools for metabolic pathway engineering. It covers applications of emerging technologies including recent research genome-wide technologies, DNA and phenotypic microarrays, and proteomics. tools for experimentally determining flux through pathways.

Full Description

Christina Smolke, who recently developed a novel way to churn out large quantities of drugs from genetically modified brewer's yeast, is regarded as one of the most brilliant minds in biomedical engineering. In this handbook, she brings together pioneering scientists from dozens of disciplines to provide a complete record of accomplishment in metabolic pathway engineering. With a wealth of cutting edge research and analysis, this work also serves as an invaluable resource for those seeking to add their own contributions. Organized by topic, this 3000 page reference is available as two volumes that can be purchased individually or as a set.FundamentalsThe first volume provides an overview of metabolic pathway engineering with a look towards the future. It discusses cellular metabolism, including transport processes inside the cell and energy generating reactions, as well as rare metabolic conversions. This volume also explores balances and reaction models, the regulation of metabolic pathways, and genome scale and multiscale modeling tools. It also covers developing appropriate hosts for metabolic engineering including the use of Escherichia coli, yeast, Bacillus subtilis, Streptomyces, filamentous fungi, annd mammalian cells using cell culture.Tool and Applications The second volume delves into evolutionary tools, including those associated with gene expression for metabolic pathway engineering. It covers applications of emerging technologies, including research on genome-wide technologies, DNA and phenotypic microarrays, and proteomics tools for experimentally determining flux through pathways. It also evaluates emerging applications for producing fine chemicals, drugs, and alternative fuels.

Contents

Volume IIntroduction and Preface toHandbook, Christina D. SmolkeProgression of biologicalsynthesis methods towards commercial relevanceThe field of metabolicengineeringAn overview of the metabolicpathway engineering handbookMetabolic engineering: lookingtowards the futureSection I.Cellular Metabolism, Vincent MartinTransport processes inside thecellEnergy generating reactionsBiosynthesis of importantbuilding blocksPolymerization of buildingblocks to macromoleculesRare metabolic conversions -harvesting diversity through natureSection II.Balances and Reaction Models, Walter van GulikGrowth nutrients and diversityMass balances, rates, andexperimentsData reconciliation and errordetectionBlack box models for growth andproduct formationMetabolic models for growth andproduct formationA thermodynamic description ofmicrobial growthSection III.Regulation of Metabolic Pathways, James LiaoRegulation of specific pathwaysRegulating of secondarymetabolismBuilding networks as assemblies of simpler controlschemesSection IV.Modeling Tools for Metabolic Engineering, Costas MaranasMetabolic flux analysisMetabolic control analysisStructure and flux analysis ofmetabolic networksConstraint-based genome-scalemodels of cellular metabolismMulti-scale modeling ofmetabolic regulationValidation of metabolic modelsSection V.Developing Appropriate Hosts for Metabolic Engineering, Jens NielsenEscherichia coli as a well-developed host for metabolic engineeringMetabolic engineering in yeastMetabolic engineering in Bacillus subtilisMetabolic engineering in StreptomycesMetabolic engineering infilamentous fungiMetabolic engineering inmammalian cells using cell cultureVolume IISection VI.Evolutionary Tools in Metabolic Engineering, Claudia Schmidt-DannertEvolutionary engineering ofindustrially important microbial phenotypesExpanding enzyme function andactivity through molecular evolutionEvolving the function of DNA andRNA regulatory regions through molecular evolutionEvolving pathways and genomesfor the production of natural and novel compoundsModels predicting optimizedevolutionary strategiesSection VII.Gene Expression Tools for Metabolic Pathway Engineering, Christina SmolkeLow-copy number plasmids asartificial chromosomesChromosomal engineeringstrategiesRegulating gene expressionthrough engineered RNA technologiesTools designed to regulatetranslational efficiencyRegulating gene expressionthrough post-translational modificationsEngineering bifunctional enzymesfor optimized metabolite transfer between sequential conversion steps Practical pathway engineering -demonstration in integrating toolsSection VIII.Applications of Emerging Technologies to Metabolic Engineering, Jay KeaslingGenome-wide technologies: DNAand phenotypic microarrays, proteomicsMonitoring and measuring themetabolomeSection IX.Future Prospects in Metabolic Engineering, Bernhard Palsson and Sang Yup LeeGenome-scale models, systemsbiology, and metabolic engineeringCell-free systems for metabolicengineeringDynamic in silico cell modelsAppendix I:Tools for Experimentally Determining Flux through Pathways, Ralf TakorsMeasuring flux by isotopelabelingTools for measuring intermediateand product formationQuantitativeanalysis of labeling dataAppendix II: Applications, BrianPfleger and Brian BrazeauCofactor regulation in biocatalytic redox reactionsMetabolic engineering for fine chemical productionMetabolic engineering for drug productionMetabolic engineering for alternative fuels