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Full Description
This multi-author volume examines the use of biochemical, molecular, biological, and recombinant DNA methodology in resolving problems in neurobiology. It surveys a range of techniques for purifying abundant mRNAs, enriching low abundance mRNAs, and using synthetic oligonucleotides for the production of complementary DNA for amplification by insertion into plasmids, and cloning and amplification in bacterial vectors.
Contents
The Construction and Identification of Recombinant DNA Probes for the Study of Gene Expression in Nervous Tissue; Peptide Hormone Gene Expression in the Brain; Molecular Biology of the Mammalian Brain; Neuron-Specific Phosphoproteins as Models for Neuronal Gene Expression; Expression of Microtubule Proteins in Brain; Modification of Gene Expression in the Mammalian Brain After Hypothermia; The Molecular Genetic Analysis of sn-Glycerol-3-Phosphate Dehyrogenase Development in Mouse Cerebellum; Molecular Biology of Myelination: Gene Expression of Myelin Basic Protein; The Bag Cell Neuron of Aplysia as a Possible Peptidergic Multi- Transmitter System: From Genes to Behavior; Vasoactive Intestinal Polypeptide: From Gene to Peptide; Index.