This book covers quality measurement, control, and improvement in combinatorial chemistry. Combinatorial chemistry has developed rapidly in the past decade, with great advances made by scientists working on analysis and purification of a large number of compounds and the analysis of polymer-bound compounds. However, formidable challenges lie ahead of today's researcher. For example, high-throughput analysis and purification technologies must be further developed to ensure combinatorial libraries are "purifiable," and "drugable." To this end, "Analysis and Purification Methods in Combinatorial Chemistry" describes various analytical techniques and systems for the development, validation, quality control, purification, and physicochemical testing of combinatorial libraries.A new volume in Wiley's "Chemical Analysis" series, this text has four parts covering: various approaches to monitoring reactions on solid support and optimizing reactions for library synthesis; high-throughput analytical methods used to analyze the quality of libraries; high-throughput purification techniques; and, analytical methods applied in post-synthesis and post-purification stages.Drawing from the contributions of respected experts in combinatorial chemistry, this comprehensive book provides coverage of applications of Nuclear Magnetic Resonance (NMR), liquid chromatography/mass spectrometry (LC/MS), Fourier Transform Infrared (FTIR), micellar electrokinetic chromatography (MEKC) technologies, as well as other analytical techniques.This eminently useful volume is an essential addition to the library of students and researchers studying or working in analytical chemistry, combinatorial chemistry, medicinal chemistry, organic chemistry, biotechnology, biochemistry, or biophysics.
Preface.Contributors.PART IOF LIBRARY SYNTHESIS.Chapter 1. Quantitative Analysis in Organic Synthesis with NMR (L. Lucas & C. Larive).Chapter 2. 19F Gel-phase NMR Spectroscopy for Reaction Monitoring and Quantification of Resin Loading (J. Salvino).Chapter 3. The Application of Single-Bead FTIR and Color Test for Reaction Monitoring and Building Block Validation in Combinatorial Library Sysnthesis(J. Cournoyer, et al.).Chapter 4. HR-MAS NMR Analysis of Compounds Attached to Polymer Supports (M. Guino & Y. de Miguel).Chapter 5. Multivariate Tools for Real-Time Monitoring and Optimization of Combinatorial Materials and Process Conditions (R. Potyrailo, et al.).Chapter 6. Mass Spectrometry and Soluble Polymeric Supports (C. Enjalbal, et al.).PART II: HIGH-THROUGHPUT ANALYSIS FOR LIBRARY QUALITY CONTROL.Chapter 7. High-Throughput NMR Techniques for Combinatorial Chemical Library Analysis (T. Hou & D. Raftery).Chapter 8. Micellar Electrokinetic Chromatography as a Tool for Combinatorial Chemistry Analysis: Theory and Applications (P. Simms).Chapter 9. Characterization of Split-Pool Encoded Combinatorial Libraries (J. Zhang & W. Fitch).PART III: Strategies and Methods for Purifying Organic Compounds and Combinatorial Libraries (J. Zhao, et al.).Chapter 11. HTP of Combinatorial Chemistry Libraries (J. Hochlowski).Chapter 12. Practical HPLC in High Throughput Analysis and Purification (H. Gumm & R. God).PART IV: ANALYSIS FOR COMPOUND STABILITY AND DRUGABILITY.Chapter 13. Organic Compound Stability in Large, Diverse Phatmaceutical Screening Collection (K. Morand & X. Cheng).Chapter 14. Quartz Crystal Microbalance in Biomolecular Recognition (M. Tseng, et al.).Chapter 15. High-Throughput Physicochemical Profiling: Potential and Limitations (B. Faller).Chapter 16. Solubility in the Design of Combinatorial Libraries (C. Lipinski).Chapter 17. High-Throughput Determination of Log D Values by LC/MS Method (J. Villena, et al.). Index.