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基本説明
初学者のみならず専門家にも最適な、生命科学者に向けた究極のオールインワンの基本ガイド。実験法の安全性・効率性・再現・トラブルシューティングを掲載したライフサイエンス研究者の為の実践的なレファレンスマニアル。重量や体積の測定からリアルタイムPCRとバイオインフォマティクスまで、基本手順と複雑な手順を網羅し、研究倫理やデータ表現についての補足記事も収録。この広範なガイドから実験の成功に必要な専門知識、スキル、理解が得られる。
The latest title from the acclaimed Current Protocols series, this Protocols provides the new researcher with the skills and understanding of the fundamental laboratory procedures necessary to run successful experiments, solve problems, and become a productive member of the modern life science laboratory. From covering the basic skills such as measurement, preparation of reagents and use of basic instrumentation to the more advanced techniques such as blotting, chromatography and real-time PCR, this book will serve as a practica reference manual for any life science researcher.
Full Description
The latest title from the acclaimed Current Protocols series, Current Protocols Essential Laboratory Techniques, 2e provides the new researcher with the skills and understanding of the fundamental laboratory procedures necessary to run successful experiments, solve problems, and become a productive member of the modern life science laboratory. From covering the basic skills such as measurement, preparation of reagents and use of basic instrumentation to the more advanced techniques such as blotting, chromatography and real-time PCR, this book will serve as a practical reference manual for any life science researcher. Written by a combination of distinguished investigators and outstanding faculty, Current Protocols Essential Laboratory Techniques, 2e is the cornerstone on which the beginning scientist can develop the skills for a successful research career.
Contents
Foreword Preface
Contributors List
Conversion Factors
Flow Chart: Application of Techniques
Emily Wiley, Claremont Colleges
Chapter 1 Volume/Weight Measurement
Unit 1.1 Volume Measurement
Andrew Zanella and Tom Davis, Claremont Colleges
Unit 1.2 Weight Measurement
Michael Guzy, Ohaus, Corp.
Chapter 2 Concentration Measurement
Unit 2.1 Spectrophotometry
Rob Morris, Ocean Optics, Inc.
Unit 2.2 Quantitation of DNA and RNA with Absorption and Fluorescence Spectroscopy
Sean R. Gallagher, UVP, LLC
Chapter 3 Reagent Preparation
Unit 3.1 Theoretical Discussion
Deb Chakravarti, Keck Graduate Institute
Unit 3.2 Practical Discussion
Deb Chakravarti, Keck Graduate Institute
Unit 3.3 Recipes
In-House
Chapter 4 Cell Culture Techniques
Unit 4.1 Aseptic Technique
Thomas Bykowski and Brian Stevenson, University of Kentucky College of Medicine
Unit 4.2 Culture of Escherichia coli and Related Bacteria
Sean P. Riley, Michael E. Woodman, and Brian Stevenson, University of Kentucky College of Medicine
Chapter 5 Sample Preparation
Unit 5.1 Centrifugation and Cell Fractionation In-House
Unit 5.2 Purification and Concentration of Nucleic Acids
Dennis H. Dowhan, University of Queensland
Chapter 6 Chromatography
Unit 6.1 Overview of Chromatography
Buddhadeb Mallik, Bulbul Chakravarti, and Deb N. Chakravarti, Keck Graduate Institute of Applied Life Sciences
Unit 6.2 Column Chromatography
Deb Chakravarti, Keck Graduate Institute
Chapter 7 Electrophoresis
Unit 7.1 Overview of Electrophoresis
Sean R. Gallagherr, UVP, LLC
Unit 7.2 Agarose Gel Electrophoresis
Jennifer A. Armstrong, The Claremont Colleges, and Joseph R. Schulz, Occidental College
Unit 7.3 SDS-PAGE
Sean Gallagher, UVP, LLC
Unit 7.4 Staining of Gels
Sean Gallagher, UVP, LLC
Chapter 8 Blotting
Unit 8.1 Overview of Blotting
Maria Cristina Negritto, Pomona College, and Glenn M. Manthey, City of Hope, Beckman Research Institute
Unit 8.2 Nucleic Acid Blotting: Southern and Northern
Laura L. Mays Hoopes, Pomona College
Unit 8.3 Protein Blotting: Immunoblotting
Sean Gallagher, UVP, LLC
Unit 8.4 Labeling DNA and Probe Preparation
Karl A. Haushalter, Harvey Mudd College
Chapter 9 Microscopy
Unit 9.1 Conventional Light Microscopy
Eric S. Cole, St. Olaf College
Unit 9.2 Immunofluorescence Microscopy
David J. Asai, Harvey Mudd College
Chapter 10 Enzymatic Reactions
Unit 10.1 Working with Enzymes
David Skrincosky and Cindy Santangelo, Worthington Biochemical Corp.
Unit 10.2 Overview of PCR
Christine D. Kuslich, Molecular Profiling Institute, Buena Chui, GE Healthcare, and Carl T. Yamashiro, Molecular Profiling Institute
Unit 10.3 Real-Time PCR
Dean Fraga, College of Wooster, Tea Meulia, Ohio Agricultural Research and Development Center, Steven Fenster, Ashland University
Chapter 11 Bioinformatics Unit 11.1 Using NCBI Blast
Nicholas A. Stover, Bradley University, and Andre R.O. Cavalcanti, Pomona College
