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Full Description
Microfluidic Enzymology, Volume 737 in the Methods in Enzymology series, presents detailed methodological descriptions and protocols covering a wide range of microfluidics-based approaches for mechanistic enzymology, high-throughput screening, and enzyme engineering campaigns. The volume includes both established methods as well as recent advances in the field. Chapters include protocols for fabricating and running microfluidic devices, state-of-the-art high-throughput droplet and spatial array-based methods, new methods for activity detection, and recent developments integrating microfluidic methods with structural biology and machine learning approaches.
Contents
Section 1: Chips and Reagents
1. Joint Methods for Fabricating Microfluidic Chips
2. A liquid handling robotic workflow for quantifying the activity of antibiotic resistance genes (ARGs) in cell-free protein synthesis
Simon Moore
Section 2: Enzyme Characterisation
3. Preparation of uniform microcrystals by droplet microfluidics for time-resolved serial crystallography
Jonathan West
4. Droplet microfluidic HDX for investigating highly dynamic proteins
Jonathan West
5. An Accessible, Low-Cost Platform for High-Throughput Microfluidic Enzyme Kinetics
Craig Markin
6. High-Throughput Data Processing
Margaux Pinney
7. DA-MEK
Polly Fordyce
8. Mass Spectrometry of Droplets
Perdita Elizabeth Barran
9. Digital Bioassay Using a Femtoliter Reactor Array Device
Hiroyuki Noji
Section 3: Enzyme Engineering
10. Confocal absorbance droplet sorting
Jean-Christophe Baret
11. Sequence-Function Data Analysis from Microfluidics Screening
Maximilian Gantz
12. Passive microfluidic system for droplet sorting based on proteolytic activity
Tomasz Kamiński
13. Directed Evolution of computationally designed enzymes on integrated devices
Richard Obexer
14. TBD
Miriam Rosenbaum
15. TBD
Guangyu Yang
16. TBD
Fuqiang Ma
Section 4: Moving beyond microfluidics (from micro to nanoscale)
17. Lithographic patterning of nanoscale enzyme arrays
Lu Shin Wong



