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Full Description
Lipids are the cell's hydrophobic metabolites, and their self-assembly into bilayers creates membranes that are critical barriers and organizing platforms in cells. Lipids are a chemically diverse set of biological molecules subject to rapid metabolism and transport, and this dynamic behavior has motivated the development of methods across multiple disciplines for their detection and manipulation. Split into three parts, the Volumes on Lipids and Membranes provide a broad snapshot of the state of the art of methods for studying biological lipids and biomembranes. This part focuses on approaches for tracking the metabolic interconversions of lipids, including the posttranslational modification of proteins with lipids (lipidation) as well as non-covalent lipid-protein interactions.
Contents
1. Assay of phospholipid scrambling
Anant K. Menon
2. Tracking lipid transport with bifunctional lipids
Andre Nadler
3. Tracking interorganelle lipid transport using genetically encoded mass tags
Benoit Kornmann
4. Molecular Dynamics Simulation of Lipid and Ligand Binding in ORD Domains
Bruno Mesmin
5. Imaging lipid droplet-organelle membrane contact sites using fluorogenic complementation
Chi-Lun Chang
6. Studying interactions of amphipathic helices with lipid droplets
Fabien Alpy
7. Metabolic labeling of to trace changes in sphingolipid metabolism
Fikadu Tafesse
8. Imaging intracellular lipid heterogeneity with fluorogen-activated coincidence sensing
Itay Budin
9. Tagging lipoproteins in mycobacteria
Jessica Seeliger
10. Probes for studying sphingolipid dynamics
Joost Holthuis
11. Measuring Flipper-TR Lifetime with Multiple Analysis Strategies
Juan Manuel Garcia-Arcos
12. Reconstitution approaches to understanding cellular lipid dynamics
Karin Reinisch
13. Prediction and characterization of the bacterial lipid handling machinery
Laura Massah Kulah Dassama
14. From Molecular Dynamics to Cryo-EM: Imaging Liposomes in Silico
Milka Doktorova
15. Genetic screens for lipid discovery in Drosophila
Raghu Padinjat
16. Imaging interorganelle membrane contact sites using dimerization-dependent fluorescent proteins
Sarah Cohen
17. Organelle-selective labeling of lipids containing azide-fatty acids
Tomonori Tamura
18. Analysis of interorganelle lipid exchange
Vytas Bankaitis
19. Reconstitution of full-length extended synaptotagmin 1 into liposomes for membrane tethering and lipid transfer assays
Xin Bian
20. Use of Small Unilamellar Vesicles to Study the Biochemical Properties of the GRAM Domain of GRAMD1
Yasunori Saheki
