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Full Description
Lipids and Membranes: Dynamics and Interorganelle Lipid Transport, Volume 728 covers lipids, a chemically diverse set of biological molecules subject to rapid metabolism and transport. This dynamic behavior has motivated the development of methods across multiple disciplines for their detection and manipulation. Split into three parts, the book's chapters include topics such as Assay of phospholipid scrambling, Tracking lipid transport with bifunctional lipids, Tracking interorganelle lipid transport using genetically encoded mass tags, Molecular Dynamics Simulation of Lipid and Ligand Binding in ORD Domains, Imaging lipid droplet-organelle membrane contact sites using fluorogenic complementation, Studying interactions of amphipathic helices with lipid droplets, and more.
Additional chapters cover Metabolic labeling of to trace changes in sphingolipid metabolism, Imaging intracellular lipid heterogeneity with fluorogen-activated coincidence sensing, Tagging lipoproteins in mycobacteria, Probes for studying sphingolipid dynamics, Measuring Flipper-TR Lifetime with Multiple Analysis Strategies, Reconstitution approaches to understanding cellular lipid dynamics, Prediction and characterization of the bacterial lipid handling machinery, From Molecular Dynamics to Cryo-EM: Imaging Liposomes in Silico, and much more.
Contents
1. Assay of phospholipid scrambling
Anant K. Menon
2. Tracking lipid transport with bifunctional lipids
Andre Nadler
3. Tracking interorganelle lipid transport using genetically encoded mass tags
Benoit Kornmann
4. Molecular Dynamics Simulation of Lipid and Ligand Binding in ORD Domains
Bruno Mesmin
5. Imaging lipid droplet-organelle membrane contact sites using fluorogenic complementation
Chi-Lun Chang
6. Studying interactions of amphipathic helices with lipid droplets
Fabien Alpy
7. Metabolic labeling of to trace changes in sphingolipid metabolism
Fikadu Tafesse
8. Imaging intracellular lipid heterogeneity with fluorogen-activated coincidence sensing
Itay Budin
9. Tagging lipoproteins in mycobacteria
Jessica Seeliger
10. Probes for studying sphingolipid dynamics
Joost Holthuis
11. Measuring Flipper-TR Lifetime with Multiple Analysis Strategies
Juan Manuel Garcia-Arcos
12. Reconstitution approaches to understanding cellular lipid dynamics
Karin Reinisch
13. Prediction and characterization of the bacterial lipid handling machinery
Laura Massah Kulah Dassama
14. From Molecular Dynamics to Cryo-EM: Imaging Liposomes in Silico
Milka Doktorova
15. Genetic screens for lipid discovery in Drosophila
Raghu Padinjat
16. Imaging interorganelle membrane contact sites using dimerization-dependent fluorescent proteins
Sarah Cohen
17. Organelle-selective labeling of lipids containing azide-fatty acids
Tomonori Tamura
18. Analysis of interorganelle lipid exchange
Vytas Bankaitis
19. Reconstitution of full-length extended synaptotagmin 1 into liposomes for membrane tethering and lipid transfer assays
Xin Bian
20. Use of Small Unilamellar Vesicles to Study the Biochemical Properties of the GRAM Domain of GRAMD1
Yasunori Saheki
