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Full Description
Methods in Enzymology series, highlights new advances in the field, with this new volume presenting interesting chapters. Each chapter is written by an international board of authors.
Contents
1. Staudinger Ligations and Related Reactions: Scope and Applications in Chemical Biology
Stefan Bräse, Juliane Ott and Ute Schepers
2. Strain-Promoted Bioorthogonal Cycloadditions to Investigate Sialyltransferases Promiscuity Towards Unnatural Sialic Acids
Frederic Friscourt and Zoeisha Chinoy
3. Practical Guidelines for the Bioorthogonal AMI-Isonitrile Ligation
Helma Wennemers
4. Nitrones and Other Dipoles in Bioorthogonal Chemistry Applications
John Paul Pezacki
5. 1,2-Aminothiol-Specific Conjugation for Dual-Color Fluorescent Labeling via Ultrafast TAMMs
Yu-Hsuan Tsai, Linlin Wang, Gaocan Dai and Sicong Yao
6. Genetic Incorporation of Bicyclononyne for Cell-Surface Protein Labeling via Tetrazine Ligation
Yu-Hsuan Tsai, Yawei Liu, Lei Zhang and Sicong Yao
7. Genetic Code Expansion for Single Noncanonical Amino Acid Incorporation into SARS-CoV-2 Omicron Spike on Amber-Free Lentiviral Particles
Maoliln Lu
8. Bioorthogonal Click-Chemistry Labeling and Visualization of Dual Noncanonical Amino Acid-Incorporated HIV-1 Env on Intact Virions
Maoliln Lu
9. Studying Bacterial Glycans with Azidosugars and Bioorthogonal Chemistry
Danielle H. Dube, Aniqa Chowdhury, Divya Bhargava and Karen Moulton
10. Bioorthogonal Tagging of DNA and RNA
Seergazhi G. Srivatsan
11. Efficient Labeling of Pathogenic Fungal Chitin with Bioorthogonal Probes
Catherine L. Grimes
12. Dendritic Cell Metabolic Labeling and Targeting
Hua Wang
13. Orthogonal Crosslinking of Monobodies Using a Genetically Encoded β-Lactam Amino Acid
Qing Lin
14. Bioorthogonal Chemical Labeling of Endogenous Receptors in the Live Mouse Brain
Itaru Hamachi
15. Organelle-Directed Metabolic Glycan Labeling
Shou-Fa Han, Shixiong Wen, Yilong Shi, Enkang Zhang and Jiahuai Han
16. Bioorthogonal Click Labeling of Proteins for Molecular Resolution Fluorescence Imaging
Markus Sauer
17. Molecular Engineering of Activatable Cyclopropenes for Bioorthogonal Labeling
Scott T. Laughlin
18. Pyrone and Pyranthione Scaffolds for Click-to-Release Biological Gas Delivery
Scott T. Laughlin
19. Click and Release through Bioorthogonal Hydroamination
Justin Kim, Tyler J Brown and Yiming Guo
20. Site-Specific Bioorthogonal Labeling of Antibodies
Lai-Xi Wang
21. inCu-click: Enabling Copper-Catalyzed Click Chemistry Inside Living Cells
Sara H. Rouhanifard
22. Bioorthogonal Photocatalytic Proximity Labeling for Quantitative Mapping of Cell-Cell Interactions
Xinyuan Fan, Yan Zhang, Nan Zhou and Fuhu Guo
23. Bioorthogonal Labeling of Sialic Acid Isomers for Imaging of Glycoconjugates Using Mass Spectrometry and Microscopy
Richard R. Drake
