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Full Description
Cell Death Part E presents the latest advances and comprehensive reviews in the rapidly evolving field of cell death. This volume explores the molecular mechanisms, regulatory pathways, and biological consequences of various forms of cell death—including apoptosis, necrosis, autophagy, and other non-canonical processes. Leading experts contribute in-depth chapters covering experimental techniques, recent discoveries, disease associations, and therapeutic implications. This book serves as an essential resource for researchers, clinicians, and students interested in understanding the complexities of cell death and its impact on health and disease.
Contents
1. Flow cytometry analysis of mitochondrial apoptotic priming with BH3 profiling on senescent cells
Joan Montero
2. Methods to study PIDDOsome and caspase 2 signaling
Valentina C. Sladky, Andreas Villunger, Felix Eichin and Rotraud Hirschberger
3. Isolation, characterization, and experimental applications of apoptotic bodies in vitro
Kristopher Sarosiek, Atomu Yamaguchi, Alejandra Garcia-Carrasco and Xingping Qin
4. Lipid ROS Measurement by Spectral Flow Cytometry based on the ratiometric dye BODIPYTM C11 581/591
Daniel Butcher, Di Yu, Naiqi Wang and Sam Nettelfield
5. CRISPR-Cas9 Screen to Identify Genes Regulating Cell Death
Dai Chao Xu and Zi Long Zheng
6. Flow cytometry-assisted discrimination of early apoptotic, late apoptotic and primary necrotic morphological markers in Saccharomyces cerevisiae
Didac Carmona-Gutierrez, Tobias Eisenberg, Frank Madeo, Martin N Odabas, Andreas Zimmermann, Katharina Kainz and Maria A Bauer
7. Cytofluorometric analysis of cell death in primary human neutrophils
Thomas Rudel and Andreas Demuth
8. Real-time tracking of cell death in human intestinal organoids: a quantitative imaging approach
James Vince
9. SHORT-TERM CULTURE OF HUMAN ENDOSCOPIC SPECIMENS FOR THE STUDY OF CHRONIC INFLAMMATION
Sonja Kallendrusch, Werner Julia, Laura Hennig, Florian Lordick, Wirtz Hubert, Frille Armin, Prill Sebastian:, Albrecht Hoffmeister, Hoang Ngoc and Cica Vissiennon
10. In vitro protocols for inducing and assessing endotoxin tolerance in human and murine macrophages
Emma M. Creagh, Aoife Smith and Mathew Choaji
11. Assessment of microglial responsiveness after irradiation in mouse organotypic hippocampus or patient-derived tissue cultures
Sonja Kallendrusch, Rapp Felicitas, Bahar Djouiai, Steffen Rosahl, Rüdiger Gerlach, Katharina Hess, Franziska Köhler, Ingo Bechmann, Frank Gaunitz, Faramarz Dehghani and Karsten Winter
