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Full Description
Proteomics in Cell Communication, offers a comprehensive exploration of the dynamic field of proteomics and its vital role in understanding cellular communication. This book includes interesting chapters on topics such as In vivo cell-type specific secretome profiling, Assays for surface antigens in extracellular vesicles using proximity labeling strategy, Cell type-specific proximity biotinylation using TurboID to identify secreted proteins from cardiomyocytes in vitro and in vivo, Native-state and cell type-specific proteomics using TurboID proximity labeling in mouse models, Adapted Luminex approach to assess phosphoproteins and cytokines released from specific cell types in the brain in vivo, and much more.
Other sections covers In-depth cell-selective proteomics and secretomics in vivo based on azidonorleucine labelling, Visualizing and profiling de novo protein synthesis in Drosophila with cell-type specificity, A Split GFP Approach for Cell-Type-Specific Protein Localization Analysis in Drosophila, Quantitative deep profiling of the cell surface proteome using the "Cell Surface Capture" technique, Proteomic profiling of extracellular fluids to identify secreted proteins from muscle and fat tissues, and much more.
Contents
1. In vivo cell-type specific secretome profiling.
toren finkel, Jie Liu and Shihui Liu
2. Assays for surface antigens in extracellular vesicles using proximity labeling strategy.
Norihiro Kotani
3. Cell type-specific proximity biotinylation using TurboID to identify secreted proteins from cardiomyocytes in vitro and in vivo. Carolina Greco
Carolina GRECO and Riccardo Doro
4. Native-state and cell type-specific proteomics using TurboID proximity labeling in mouse models
Srikant Rangaraju, Prateek Kumar, Dilpreet Kour, Rashmi Kumari, Wooyoung E. Jang, Nicholas T. Seyfried, Levi B. Wood and Matthew J. Rowan
5. Adapted Luminex approach to assess phosphoproteins and cytokines released from specific cell types in the brain in vivo.
Sara Bitarafan, Levi B. Wood, Srikant Rangaraju, Brendan Tobin and Claudia Espinosa-Garcia
6. In-depth cell-selective proteomics and secretomics in vivo based on azidonorleucine labelling.
Jonathan Johannes Swietlik and Felix Meissner
7. Visualizing and profiling de novo protein synthesis in Drosophila with cell-type specificity.
Ian Martin, Stefanny Villalobos-Cantor, Ruth Barrett, Alec Condon, Alicia Arreola-Bustos, Kelsie Rodriguez and Michael Cohen
8. A Split GFP Approach for Cell-Type-Specific Protein Localization Analysis in Drosophila.
Daichi Kamiyama. and Melissa Inal
9. Quantitative deep profiling of the cell surface proteome using the "Cell Surface Capture" technique
Arun Wiita
10. Proteomic profiling of extracellular fluids to identify secreted proteins from muscle and fat tissues
Bruce Spiegelman and Melanie Mittenbuhler
11. A Cell Culture-Based Method for Interrogating Muscle to Liver Communication via Secreted Proteins
Kevin KORONOWSKI and Ioannis Tsialtas
12. SILAC-based assessment of protein palmitoylation by metabolic labelling and click-chemistry.
Miguel Martin Perez, Anke Vandekeere, Sarah Maria Fendt and Salvador Aznar Benitah
13. Separating the wheat from the chaff: Determination of confidently secreted proteins by including information of their cellular abundance
Gereon Poschmann, Ronja Marie Schwermer and Kai Stühler
