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Full Description
Super-Resolution Nanoscopy for Protein Imaging decodes the intricate world of protein visualization using cutting-edge nanoscopic techniques. This comprehensive guide is crafted to equip master students, PhD candidates, and early-stage researchers with essential knowledge and practical insights into advanced protein imaging technologies. The book covers foundational principles, innovative methodologies, and real-world applications, providing a complete resource for mastering the complexities of protein imaging. This book offers a unique compilation of diverse nanoscopic methods, overcoming the limitation of technique-specific texts currently available. By collecting scattered information into a single volume, it delivers an in-depth exploration of imaging techniques, from Atomic Force Microscopy (AFM) to Electron Microscopy (EM), and Single Molecule Fluorescence Imaging. Each section delves into instrumentation, sample preparation, data analysis, and the latest developments, making it an indispensable reference for understanding how to visualize proteins in their natural environments. Designed to inspire innovation and facilitate practical application, Super-Resolution Nanoscopy for Protein Imaging addresses key areas such as the dynamic analysis of protein structure and function, the strengths and limitations of various imaging techniques, and their applications in biological research. Real-world examples and case studies throughout the book provide a comprehensive guide for scientists seeking to unlock new discoveries in protein imaging. Ideal for researchers, method developers, and protein origami pioneers, this volume bridges the gap between theoretical knowledge and practical implementation, making it a must-read for anyone looking to excel in the rapidly evolving field of protein imaging technologies.
Contents
Section 1 - Nanoscopy: An Introduction
1. Introduction to Nanoscopy for Protein Imaging
2. Nanoscopy for Dynamic Analysis of Protein Structure and Function
Section 2 - Atomic Force Microscopy (AFM)
3. Instrumentation
4. Sample Preparation
5. Image Processing and Automation Aspects
6. Latest developments in Live Protein Imaging with AFM
Section 3 - Electron Microscopy
7. Instrumentation
8. Sample Preparation
9. Image Processing and Automation Aspects
10. Ultrahigh resolution of Imaging of Protein
Section 4 - Single Molecule Fluorescence Imaging and other Optical Imaging Methods
11. Instrumentation
12. Sample Preparation
13. Image Processing and Automation Aspects
14. A Review various of types of enzymes imaged using optical imaging methods
