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Full Description
CRISPR-Cas Systems for RNA and Genome Editing, Volume 711 in this ongoing series, highlights new advances in the field, with this new volume presenting interesting chapters on topics such as Massively parallel genomic perturbations with multi-target CRISPR interrogates Cas9 activity and DNA repair at endogenous sites, Genome oligopaint via local denaturation fluorescence in situ hybridization, Biochemical reconstitution of a type I-B CRISPR-associated transposon, Visualizing the conformational landscape of CRISPR-Cas9 through kinetics-informed structural studies, and much more.
Additional sections cover Analysis of metal-dependent DNA nicking activities by Cas endonucleases, Biochemically characterizing the accessory proteins and ribonucleoprotein complexes of Type IV CRISPR systems, Prime editing in bacteria with BacPE, Preparation of high-purity CRISPR-based DNA base editor RNPs, Bacterial directed evolution of CRISPR base editors, Cloning and validating systems for high throughput molecular recording, Using CRISPR-Cas13 for viral nucleic acid detection, and more.
Contents
1. Massively parallel genomic perturbations with multi-target CRISPR interrogates Cas9 activity and DNA repair at endogenous sites
TJ Ha
2. Genome oligopaint via local denaturation fluorescence in situ hybridization
TJ Ha
3. TBD
Osamu Nureki
4. Biochemical reconstitution of a type I-B CRISPR-associated transposon
Leifu Chang
5. TBD
Yibei Xiao
6. Visualising the conformational landscape of of CRISPR-Cas9 through kinetics-informed structural studies
David W. Taylor
7. Analysis of metal-dependent DNA nicking activities by Cas endonucleases
Dipali G. Sashital
8. Biochemically characterizing the accessory proteins and ribonucleoprotein complexes of Type IV CRISPR systems.
Ryan Jackson
9. HYER
Junjie (Gogo) liu
10. focused on the purification and characterization of gRAMP, as well as its application in GFP knockdown in E. coli.
Chunyi Hu
11. TBD
Peter Fineran
12. Cas12a2 ?
Chase Beisel
13. Prime editing in bacteria with BacPE
Quanjiang Ji
14. TBD
Jia Chen
15. Preparation of high-purity CRISPR-based DNA base editor RNPs
Audrone Lapinaite
16. Bacterial directed evolution of CRISPR base editors
Shannon Miller
17. Cloning and validating systems for high throughput molecular recording
Michelle Chan
18. TBD
Samagya Banskota
19. Using CRISPR-Cas13 for viral nucleic acid detection
Cameron Myhrvold
20. CRISPR-based epigenetic editing for programmable gene silencing in mammalian cells
James Nuñez
21. Genome editing with programmable base editors in human cells" - explaining how to pick out and validate BE:gRNA combinations for a given target, with tips and tricks for maximizing editing efficiencies
Alexis Komor
22. High-throughput evaluation of genetic variants using base/prime editing sensor libraries
Francisco J. Sánchez Rivera
23. TBD
Jia Chen
